基于室温离子液体的有序多孔金膜葡萄糖传感器  

Preparation of glucose sensor based on three-dimensional ordered macroporous gold film and room temperature ionic liquid

作　　者：陈晓君[1,2] 宣婕 姜立萍[1] 朱俊杰[1] 

机构地区：[1]生命分析化学教育部重点实验室、南京大学化学化工学院,南京210093 [2]南京工业大学理学院,南京210009

出　　处：《中国科学（B辑）》2009年第11期1529-1535,共7页Science in China(Series B)

基　　金：国家自然科学重点基金(批准号:20635020)资助

摘　　要：将1-丁基-3-甲基咪唑四氟硼酸盐([BMIm][BF4])、N,N-二甲基甲酰胺(DMF)与葡萄糖氧化酶(GOD)的混合物修饰于三维有序大孔(3DOM)金膜电极上,构建了一种新型的葡萄糖传感器.固定的GOD在pH7.0的磷酸缓冲液(PBS)中展现出一对可逆性好的氧化还原峰,这归因于GOD的活性中心黄素腺嘌呤二核苷酸(FAD)的直接电化学行为.研究表明,离子液体(IL)、DMF以及3DOM金膜对GOD的直接电化学都起到了重要的作用.3DOM金膜修饰电极作为基底提高了酶的负载量,加速了GOD与电极表面的电子传递;IL的应用增加了固定GOD的电化学活性;DMF与IL、GOD的协同作用更好地保持了GOD的生物活性.固定在电极表面的GOD对葡萄糖显示出良好的催化性能,其检测线性范围为10～125nmol/L,检测限为3.3nmol/L(S/N=3),酶催化反应的表观米氏常数Km为0.018mmol/L.A novel type of glucose sensor was fabricated based on a glucose oxidase （GOD）-N,N-dimethtylformamide （DMF）-[BMIm][BF4] composites modified three-dimensional ordered macroporous （3DOM） gold film electrode. The immobilized GOD exhibits a pair of well-defined reversible peaks in 50 mmol/L pH 7.0 phosphate buffer solutions （PBS）, which could be attributed to the redox of flavin adenine dinucleotide （FAD） in GOD. The research results show that ionic liquid （[BMIm][BF4]）, DMF and 3DOM gold film are crucial for GOD to exhibit a pair of stable and reversible peaks. It is believed that the large active area of 3DOM gold film can increase the amount of immobilized GOD. Simultaneously, the application of IL enhances the stability of GOD and facilitates the electron transfer between GOD and electrode. The synergetic effect of DMF can help the GOD to maintain its bioactivity better. GOD immobilized on the electrode exhibits favorable electrocatalytic property to glucose, and the prepared sensor has a linear range from 10 to 125 nmol/L with a detection limit of 3.3 nmol/L at a signal-to-noise ratio of 3a. The apparent Km （Michaelis-Menten constant） for the enzymatic reaction is 0.018 mmol/L

关 键 词：离子液体 葡萄糖氧化酶 三维有序大孔金膜 直接电化学 电化学催化 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]