葡萄糖氧化酶在羟基磷灰石/Nafion复合膜修饰电极上的直接电化学及其对葡萄糖的生物传感  

Direct electrochemistry of glucose oxidase on hydroxyapatite/Nafion composite film modified electrode and its application for glucose biosensing

作　　者：马荣娜[1] 王斌[1] 刘燕[1] 李静[1] 赵倩[1] 王国涛[1] 贾文丽[1] 王怀生[1] 

机构地区：[1]聊城大学化学化工学院分析化学研究所,聊城252059

出　　处：《中国科学（B辑）》2009年第11期1544-1550,共7页Science in China(Series B)

基　　金：国家自然科学基金(批准号:20475024,20775031);山东省“泰山学者”建设工程专项经费资助

摘　　要：将葡萄糖氧化酶固定于羟基磷灰石(HAp)-Nafion复合膜,构建了高灵敏、高选择性的葡萄糖传感器.羟基磷灰石和Nafion良好的协同作用,可以有效地提高传感器的稳定性与灵敏度.实验结果表明:固定在复合膜修饰电极上的葡萄糖氧化酶呈现出一对较好的近乎可逆的氧化还原峰,并且对葡萄糖的氧化有良好的催化作用,同时消耗溶解氧,从而导致溶解氧还原峰的降低.在-0.8V处,随葡萄糖浓度的增加,葡萄糖氧化酶催化葡萄糖氧化时消耗溶解氧的量增加,溶解氧还原电流逐渐降低,因此该修饰电极可以作为葡萄糖传感器实现对葡萄糖的高灵敏检测.在0.12～2.16mmol·L-1浓度范围内,溶解氧还原电流的降低与葡萄糖的浓度成正比,据此可以测定出溶液中葡萄糖的浓度,该传感器的检出限和灵敏度分别为0.02mmol·L-1(S/N=3)和6.75mA·L-mol-1.因此,HAp-Nafion复合膜为酶的固定和直接电化学研究提供了一个新的有效平台,在构建新型无试剂葡萄糖传感器方面具有较大的应用前景.A novel glucose biosensor was constructed by immobilizing the glucose oxidase （GOD） on a hydroxyaparite （HAp）/Nation composite film modified glassy carbon electrode （GCE） and applied to the highly selective and sensitive determination of glucose. With the cooperation of HAp and Nation, the composite film played an important role in enhancing the stability and sensitivity of the biosensor. The results demonstrated that the GOD adsorbed onto the HAp/Nafion composite tilm exhibits a pair of well-defined nearly reversible redox peaks and fine catalysis to the oxidation of glucose companied with the consumption of dissolved oxygen. Based on the decrease of the reduction current of dissolved oxygen at the applied potential of-0.80 V （vs. SCE） upon the addition of glucose, the concentration of glucose could be detected sensitively and selectively. The decreased reduction current was linear with the concentration of glucose in the range of 0.12 - 2.16 mmol·L^-1. The detection limit and sensitivity were 0.02 mmol·L^-1 （S/N = 3） and 6.75 mA·L·mol^-1, respectively. All the results demonstrated that HAp/Nation composite film provides a novel and efficient platform for the immobilization of enzymes and realizes the direct electrochemistry. The composite materials should have potential applications in the fabrication of third-generation biosensors.

关 键 词：葡萄糖氧化酶 葡萄糖 羟基磷灰石 NAFION 直接电化学 生物传感器 

分 类 号：O646.54[理学—物理化学] Q554[理学—化学]