Modelling of a DNA packaging motor  

作　　者：钱钧 谢平 薛晓光 王鹏业 

机构地区：[1]College of Physics Science,Nankai University [2]Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences [3]Department of Physics,Renmin University of China

出　　处：《Chinese Physics B》2009年第11期4852-4864,共13页中国物理B（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant Nos 10834014 and 10674173);the National Basic Research Program of China (Grant No 2009CB930704)

摘　　要：During the assembly of many viruses, a powerful molecular motor packages the genome into a preassembled capsid. The Bacillus subtilis phage φ29 is an excellent model system to investigate the DNA packaging mechanism because of its highly efficient in vitro DNA packaging activity and the development of a single-molecule packaging assay. Here we make use of structural and biochemical experimental data to build a physical model of DNA packaging by the φ29 DNA packaging motor. Based on the model, various dynamic behaviours such as the packaging rate, pause frequency and slip frequency under different ATP concentrations, ADP concentrations, external loads as well as capsid fillings are studied by using Monte Carlo simulation. Good agreement is obtained between the simulated and available experimental results. Moreover, we make testable predictions that should guide future experiments related to motor function.During the assembly of many viruses, a powerful molecular motor packages the genome into a preassembled capsid. The Bacillus subtilis phage φ29 is an excellent model system to investigate the DNA packaging mechanism because of its highly efficient in vitro DNA packaging activity and the development of a single-molecule packaging assay. Here we make use of structural and biochemical experimental data to build a physical model of DNA packaging by the φ29 DNA packaging motor. Based on the model, various dynamic behaviours such as the packaging rate, pause frequency and slip frequency under different ATP concentrations, ADP concentrations, external loads as well as capsid fillings are studied by using Monte Carlo simulation. Good agreement is obtained between the simulated and available experimental results. Moreover, we make testable predictions that should guide future experiments related to motor function.

关 键 词：DNA translocation PAUSE SLIP molecular motor 

分 类 号：Q939.4[生物学—微生物学]