卡介菌多糖核酸对特应性皮炎外周血CLA^+T细胞表达不同细胞因子的影响  

Effect of PNCG on the Expression of CLA^+T Cell Derived Cytokines in Atopic Dermatitis

作　　者：张丽[1] 齐瑞群[1] 孙艳[1] 陈国红[1] 任宏伟[1] 陈洪铎[1] 高兴华[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中国医科大学学报》2009年第10期764-766,共3页Journal of China Medical University

基　　金：教育部长江学者和创新团队发展计划基金资助项目(IRT0760);辽宁省创新团队基金资助项目(2007T186)

摘　　要：目的探讨卡介菌多糖核酸(PNCG)治疗特应性皮炎(AD)前后,患者外周血人皮肤淋巴细胞相关抗原(CLA)阳性的T淋巴细胞表达不同细胞因子的频数及其与疾病严重程度的相关性。方法以双盲、随机、交叉对照方法应用卡介菌多糖核酸及安慰剂治疗AD患者,应用流式细胞术检测治疗前后AD患者外周血CLA+T淋巴细胞表达不同细胞质内细胞因子(包括IL-4、IL-5、IFN-γ、TNF-α)的频数;利用AD皮损面积严重度指数积分(ADASIS)法,获取治疗前后AD患者病情严重程度积分。结果与安慰剂相比,卡介菌多糖核酸治疗后AD患者外周血中CLA+T淋巴细胞内表达IL-5的频数明显下降;CLA+T淋巴细胞内表达IFN-γ明显升高;卡介菌多糖核酸治疗后患者ADASIS积分下降。结论卡介菌多糖核酸作为一种免疫调节药物,可通过纠正CLA+T淋巴细胞亚群的免疫失衡状态治疗特应性皮炎。Objective To investigate the influence of polysaccharide and nucleoside extract from bacille Calmett-Guern （PNCG） on the frequency of peripheral cutaneous lymphocyte-associated antigen positive （CLA ＋ ） T lymphocyte derived lymphokines in atopic dermatitis （AD） and to explore their associations with the disease severity. Methods A randomized, double blind and placebo cross-over control method was used to treat the AD patients. Before and after the treatment, flow cytometry was used to measure the frequencies of IL-4, IL-5, IFN-γ,TNFα positive CLACF cells. Disease severity was evaluated by atopie dermatitis area and severity index score （ADASIS）. Results After the PNCG treatment,the frequency of IL-5＋ CLA＋T cell decreased significantly,while IFN-γ increased,compared with the placebo con- trol group. PNCG could improve the ADASIS. Conclusion PNCG may treat AD through restoring the immune balance of CLA＋T cells.

关 键 词：特应性皮炎 卡介菌多糖核酸 人皮肤淋巴细胞相关抗原 细胞因子 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]