不同产地温郁金遗传多样性的SRAP分析  

Study on Genetic Diversity of Curcuma wenyujin from Various Habitats by SRAP

作　　者：冷春鸿[1] 陶正明[1] 吴志刚[1] 林新春[2] 娄永峰[2] 姜程曦[1] 

机构地区：[1]浙江省亚热带作物研究所,浙江温州325005 [2]浙江林学院浙江省现代森林培育技术重点实验室,浙江临安311300

出　　处：《中药材》2009年第10期1507-1510,共4页Journal of Chinese Medicinal Materials

基　　金：温州市重点科技计划项目(N20070023)

摘　　要：目的:构建不同产地温郁金的DNA指纹图谱,探讨利用SRAP技术在温郁金亲缘关系研究中的可行性。方法:采用SRAP分子标记对不同产地温郁金样品进行基因组DNA多态性分析。结果:筛选出5对引物组合,构建了不同产地温郁金的SRAP指纹图谱,聚类结果表明,不同产地温郁金的遗传差异不明显。结论:SRAP技术可以为温郁金品种的保护与利用提供一定的理论依据。Objective：The DNA fingerprints of Curcuma wenyujin from various habitats were generated by using SRAP markers to find the feasibility in analyzing their relationship. Methods： The DNA polymorphism of Curcuma wenyujin from various habitats were detected by SRAP molecular markers. Results： We had screened five pairs of primer combinations, and built the DNA fingerprints of Curcuma wenyujin from various habitats. The phylogenetic clustering results revealed that the genetic difference in Curcuma wenyujin from various habitats were little. Conclusion ： SRAP markers may offer some evidence for protecting and exploiting of Curcuma wenyuji.

关 键 词：温郁金 遗传多样性 SRAP 

分 类 号：R282.2[医药卫生—中药学]