穿心莲内酯和脱水穿心莲内酯表观油水分配系数的测定及pH值对其的影响  

Determination of Apparent Oil-water Partition Coefficient of Andrographolide and Dehydroandrographolide and Effect of pH on Them

作　　者：姚媛[1] 廖琼峰[1] 曾丽英 张蕾[1] 马燕[1] 曾元儿[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州市越秀区妇幼保健院,广东广州510055

出　　处：《中药材》2009年第10期1610-1612,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30600823)

摘　　要：目的:测定穿心莲内酯和脱水穿心莲内酯在正辛醇-水和正辛醇-缓冲液体系中的表观油水分配系数,为体内吸收研究提供参考。方法:采用摇瓶法测定穿心莲内酯和脱水穿心莲内酯的表观油水分配系数,采用HPLC法测定穿心莲内酯和脱水穿心莲内酯的浓度。结果:37℃下穿心莲内酯和脱水穿心莲内酯表观油水分配系数分别为3.90(log Papp=0.59)和19.75(log Papp=1.30)。结论:pH对穿心莲内酯和脱水穿心莲内酯表观油水分配系数有一定影响。在一定范围内,pH值增加可使穿心莲内酯和脱水穿心莲的表观油水分配系数减小。穿心莲内酯在pH6的磷酸盐缓冲液时表观油水分配系数最大[2.62(log Papp=0.42)],脱水穿心莲内酯则在pH5时达到最大[25.19(log Papp=1.40)]。Objective：To determine the apparent n-octanol-water/ buffer partition coefficient of andrographolide and dehydroan- drographolide. Methods ： The apparent n-octanol-water/buffer partition coefficients of andrographolide and dehydroandrographolide were measured by shaking flask method. The concentrations of andrographolide and dehydroandrographolide were analyzed by HPLC method. Results ： The Papp of andrographolide and dehydroandrographolide were 3.90 （ log Papp = 0. 59 ） and 19. 75 （ log Papp = 1.30） in water at 37℃, respectively. Conclusions：The apparent n-octanol-buffer partition coefficients of andrographolide and dehydroandrographolide are influenced by pH, and the higher pH may decrease the apparent n-octanol-buffer partition coefficients of them. Andrographolide has the highest partition coefficient in pH6, and dehydroandrogapholide in pH5.

关 键 词：穿心莲内酯 脱水穿心莲内酯 表观油水分配系数 

分 类 号：R285[医药卫生—中药学]