益肾化瘀方对肾间质纤维化大鼠肾组织中CTGF蛋白表达的影响  

Influence of Yishen Huayu Formula on expression of connective tissue growth factor in rat with renal interstitial fibrosis

作　　者：张琳琪[1] 高志卿[1] 李伟明[2] 

机构地区：[1]河南中医学院第一附属医院,郑州450000 [2]河南中医学院第三附属医院,郑州450008

出　　处：《中华中医药杂志》2009年第11期1500-1502,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:动态观察肾间质纤维化大鼠肾组织中结缔组织生长因子(CTGF)蛋白的表达变化以及益肾化瘀方对其影响,探讨益肾化瘀方治疗肾间质纤维化的部分机制。方法:将150只大鼠随机分为假手术组、模型组、益肾化瘀方高剂量组、益肾化瘀方低剂量组、洛汀新组,采用单侧输尿管结扎(UUO)的方法建立肾间质纤维化大鼠模型;分别于UUO术后3、7、14、21、28d观察模型大鼠肾组织中CTGF蛋白的表达变化,采用Western-blot方法,以β-actin为内参,测定CTGF蛋白的相对表达量。结果:与假手术组相比,模型组大鼠肾组织CTGF蛋白表达于7d后出现不同程度的增高(P<0.01);用药后至第7d,与模型组相比,各给药组大鼠肾组织CTGF蛋白表达均有所降低(P<0.05或P<0.01),益肾化瘀方高剂量组优于益肾化瘀方低剂量组和洛汀新组(P<0.05或P<0.01)。结论益肾化瘀方可以通过降低肾组织中CTGF蛋白表达,从而防治肾间质纤维化。Objective： To investigate the protective influence and mechanism of Yishen Huayu Formula on the expression of connective tissue growth factor （CTGF）in the rat with renal interstitial fibrosis. Methods： 150 rats were randomly divided into sham-opration group, model group, Lotensin treatment group, Yishen Huayu high low dose treatment groups. The rat models with renal interstitial fibrosis were induced by unilateral ureteral obstruction. The expressions of CTGF in the kidneys were observed on 3rd, 7th, 14th, 21st, 28th day. The alterations of renal the expressions of CTGF in the kidneys were observed with the β -actin as intrinsic control. Results： Compared with the control group, the expression of CTGF increased significantly in model group（P〈0.01）. Compared with model group, all the indicators mentioned above in treatment groups decreased（P〈0.05 or P〈0.01）. Yishen Huayu high dose group was better than Yishen Huayu low dose group and Lotensin group in decreasing the expression of CTGF（P〈0.05 or P〈0.01）. Conclusions： Yishen Huayu can treat and prevent renal interstitial fibrosis.

关 键 词：益肾化瘀方 大鼠 肾间质纤维化 结缔组织生长因子 

分 类 号：R285.5[医药卫生—中药学]