小檗碱对HMGB1胞外释放的影响  

Effect of berberine on extracellular release of HMGB1

作　　者：陈国千[1] 吴国荣[1] 赵琪[1] 郭继忠[1] 袁劲松[1] 帅朝霞[1] 徐明[2] 

机构地区：[1]无锡市人民医院医学检验科,无锡214023 [2]卫生部寄生虫病预防与控制技术重点实验室,无锡214064

出　　处：《中华中医药杂志》2009年第11期1530-1532,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省自然科学基金资助项目(No.BK2006027);江苏省中医药局科研项目(No.H05178)

摘　　要：目的:研究小檗碱对高迁移率族蛋白B1(HMGB1)胞外释放的影响及其机制。方法:观察小檗碱对内毒素脂多糖激活后培养巨噬细胞株RAW264.7HMGB1基因表达和释放的作用,及对内毒素血症小鼠血清HMGB1水平和存活率的影响。结果:小檗碱(100μmol/L)明显抑制内毒素脂多糖激活后巨噬细胞HMGB1的mRNA表达和胞外释放(P<0.05)。内毒素血症小鼠腹腔注射小檗碱(5mg/kg)后,血清HMGB1水平降低,96h后小鼠存活率由28%提高至56%。结论:小檗碱具有抑制细胞HMGB1释放和保护内毒素血症小鼠作用。Objective： To study the effect of berberine on extracellular release of high mobility group box 1 （HMGB1） and its mechanism. Methods： The effect of berberine on release of HMGB1 and expression of HMGB1 mRNA were investigated in cultured macrophage-like RAW 264.7 cells after stimulation with lipopolysaccharide. The effect of berberine on serum HMGB 1 level and survival rate of endotoxemia mice was also studyed. Results： Berberine（100pmol/L） markedly suppressed the release of HMGB1 and expression of HMGB 1 mRNA from lipopolysaccharide-stimulated macrophages（P 〈 0.05）. Serum HMGB1 level decreased, and the survival rate elevated from 28% to 56% at 96 hours, after endotoxemia mice were injected with berberine（5mg/ kg） intraperitoneally. Conclusion： Berberine was an effective inhibitor of HMGB 1 release, and confered protection against endotoxin lethality.

关 键 词：HMGB1 小檗碱 巨噬细胞 内毒素血症 

分 类 号：R285[医药卫生—中药学]