细菌纤维素的体内降解及其组织相容性  

Degradation and Histocompatibility of Bacterial Cellulose

作　　者：陈艳梅[1] 奚廷斐[1,2,3] 郑琪[4] 郑裕东[1] 万怡灶[5] 高川[5] 

机构地区：[1]北京科技大学材料科学与工程学院,北京100083 [2]北京大学前沿交叉学科研究院,北京100871 [3]温州医学院信息与工程学院,浙江温州325035 [4]中国药品生物制品检定所,北京100050 [5]天津大学材料学院,天津300072

出　　处：《科技导报》2009年第21期61-66,共6页Science & Technology Review

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB936101)

摘　　要：为了研究细菌纤维素(BC)植入皮下组织后对其周围组织细胞产生的影响,以及由于周围细胞的长入和组织液中相关成分的直接接触,使材料在体内复杂环境下所发生的物理结构变化,以组织工程用支架基体材料细菌纤维素为研究对象,对这种材料的体内降解行为和体内组织相容性进行研究。选取成熟的Wistar大鼠,采取背部皮下植入的方式,将BC植入一定时间后,对取出的BC清洗干净后进行材料学检测分析,对植入部位的组织进行透射电镜观察分析。对取出的材料进行结构和形貌表征可知,随着植入时间的延长,在细胞和组织液的综合作用下,BC分子链中部分C—O—C键断裂,分子间结合力降低,结晶度逐渐下降。因此,BC在体内复杂环境的影响下会发生轻微降解。BC与皮下组织有较好的生物相容性,材料周围组织细胞状态良好,能长入材料30μm处的空间网络结构中,并且伴有血管生成。Bacterial Cellulose （BC） used in tissue engineering is studied in this paper, with the focus on the effects of bacterial cellulose on rhagiocrine cell and the structural changes of materials owing to tissue cells entering. To study the in vivo degradation and histocompatibility of BC, BC with the size of 5 mm×10 mm×1mm was implanted in the subcutaneous tissue back of mature Wistar rats. After a period of time, BC was taken out and cleaned out with Sodium Dodecyl Sulfate （SDS）, Phosphate Buffer Solution （PBS） and distilled water. And then the net BC taken out in different times was detected and analyzed by XRD, SEM and FTIR. At the same time, the corresponding subcutaneous tissue was fixed and observed under Transmission Electron Microscope （TEM）. The interface of materials and organization tissue was Observed by TEM to investigate the condition of histiocyte growing into 3D network of materials. The materials were taken out from the tissue after BC was implanted for several months. It is found that the crystallinity of BC is stepped down and C--O bond characteristic absorption peak broadened. Moreover, calcium phosphate salts were found deposited on the nano-fibril of BC after implanted for 6 months. Bacterial cellulose had good histocompatibility with subcutaneous tissue of back. Fibroblasts as well as blood vessel could migrate up to 30 μm into the fibrous network after 6 months of implant.

关 键 词：细菌纤维素 降解 组织相容性 

分 类 号：R318.08[医药卫生—生物医学工程]