葛根素对脑缺血大鼠神经元凋亡及脑源性神经营养因子的影响  

Influence of puerarin on cell apoptosis and bd neurotrophlc factor after cerebral ischemia in rats

作　　者：韩江全[1] 李均[1] 李官成[2] 周晓兰[3] 朱选平[1] 梁恒[1] 李继中[1] 林冬融[1] 赖敏[1] 

机构地区：[1]遵义医学院第五附属医院神经内科,广东珠海519100 [2]遵义医学院珠海校区中心实验室,广东珠海519100 [3]遵义医学院第五附属医院检验科,广东珠海519100

出　　处：《西部医学》2009年第11期1844-1846,共3页Medical Journal of West China

摘　　要：目的观察葛根素(Pue)对局灶脑缺血大鼠脑源性神经营养因子(BDNF)表达的影响,进一步探讨葛根素的神经保护作用。方法制作大鼠大脑中动脉闭塞(MCAO)模型,30只SD雄性大鼠被随机分为假手术组、缺血组、Pue治疗组。应用TTC染色观察梗死体积,TUNEL染色检测凋亡细胞数,免疫组化方法观察Pue治疗组及脑缺血组BDNF阳性细胞数,并进行图像分析。结果与假手术组相比,缺血组及Pue治疗组BDNF阳性细胞均显著增加。且Pue治疗组阳性细胞数又显著高于缺血对照组(P<0.05)。结论Pue的神经保护作用可能与其能提高内源性BDNF的表达水平有关。Objective To observe the influence of Puerarin（Pue） on cell apoptosis and expression of brain-derived neurotrophic factor （BDNF） after focal cerebral isehemia in rats. Methods Animal model was established by middle cerebral artery occlusion （MCAO） in rats. 40 healthy male Sprague Dawley （SD） rates were randomly divided into 3 groups, including sham operation group, cerebral ischemia group and pue treatment group. The ischemic zone was observed with tetraphenyl tetrzolium chloride （TTC） staining. The nerve cells apoptosis was detected with erminal deoxynucleotidyl transferase. BDNF positive neurons were examined with immunohistochemistry. Results Compared with the cerebral ischemia group, the size of the infarct and the number of the nerve cells apoptosis in Pue treatment group were remarkably decreased （P〈0.01）, while expression of BDNF was obviously increased （P〈0.05）. Conclusion The neuroprotection of puerarin against cerebral ischemia may be associated with that Pue increased the expression level of BDNF after cerebral ischemia in rats.

关 键 词：葛根素 脑源性神经营养因子 脑缺血 免疫组织化学 大鼠 

分 类 号：R931.5[医药卫生—生药学] R743.3[医药卫生—药学]