拟南芥隐花色素突变体抑制子的筛选及其表型分析  

Screening and Phenotypic Analysis of a Suppressor of Cryptochromes Mutant in Arabidopsis

作　　者：陈福禄[1] 李宏宇[1] 林辰涛[1,2] 傅永福[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]美国加州大学分子细胞与发育生物学系,美国洛杉矶90095

出　　处：《中国农业科技导报》2009年第3期93-97,共5页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30570162)资助

摘　　要：隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性。拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能。cry1cry2双突变体在长日条件下比野生型晚开花。采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D(suppressor of cry1cry2#17-Dominant)。TAIL-PCR结果表明,scc17-D突变体的T-DNA插入在第一条染色体上的At1g25440和At1g25450两个基因之间。scc17-D突变体表现出植株矮化、叶片变小、果荚变小和子叶表皮细胞形状发生改变等性状。Cryptochromes are flavoproteins that share similarity with the sequence of amino acids in DNA photolyase but lack photolyase activity. Among members of cryptochromes gene family in Arabidopsis thaliana, cryptochromel （CRY1） and CRY2 primarily mediate blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation, respectively, although their functions do overlap. The crylcry2 double mutant flowered later than wild-type under long day conditions. Through activation tagging, we identified an early flowering mutant called scclT-D（suppressor of crylcry2#17-Dominant）. TAIL-PCR results showed that a T-DNA insertion located between Atlg25440 and Atlg25450 in the first chromosome is responsible for the sccl7-D mutant. The sccl7-D mutant displayed dwarfism, reduction in leaf size, reduced length of siliques, and the altered shape of cotyledon pavement cells.

关 键 词：拟南芥 隐花色素 光周期 激活标签 开花 

分 类 号：Q754[生物学—分子生物学]