甲状腺癌转移性淋巴结的红外光谱研究  

Investigation on Fourier Transform Infrared Spectrum of Metastatic Lymph Node in Thyroid Cancer

作　　者：刘亚奇[1] 高梅娟[1,2] 徐怡庄[3] 孙启刚[1] 张小青[1] 徐智[1] 张元福[3] 吴瑾光[3] 周孝思[1] 凌晓锋[1] 

机构地区：[1]北京大学第三医院普通外科,北京100191 [2]北京工业大学校医院,北京100124 [3]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2009年第11期2917-2921,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50673005);北京市自然科学基金项目(2072020)资助

摘　　要：联合运用衰减全反射(attenuated total reflectance,ATR)探头与FTIR光谱仪,测定了新鲜离体的甲状腺癌转移性颈部淋巴结20枚和非转移性淋巴结69枚的FTIR光谱,比较两组光谱13个谱带的峰位及相对峰强等28个指标,找出甲状腺癌转移性淋巴结光谱的特征。与非转移性淋巴结相比,转移性淋巴结光谱中与蛋白、脂质、糖类、核酸相关的谱带均发生了明显的变化,具体如下:(1)与蛋白质相关的谱带的相对峰强I3280/I1460(P=0.029),I1640/I1460(P=0.019),I1546/I1460(P=0.004)均显著升高;(2)脂类相关的相对峰强I1743/I1460显著降低(P=0.026),而I1400/I1460则明显增高(P=0.001);(3)与糖类相关的1165与1120cm-1处峰位P1165(P=0.019),P1120(P=0.000)发生明显蓝移(向高波数移动),相对峰强I1165/I1460显著降低(P=0.006);(4)与核酸相关的1085cm-1处峰位P1085发生明显地红移(向低波数移动)(P=0.029);(5)其他指认未明的谱带的相对峰强I1303/I1460(P=0.008),I1303/I1240(P=0.039)均明显升高。与非转移性淋巴结光谱相比较,甲癌转移的淋巴结光谱在多个谱带的峰位或相对峰强方面存在显著的差异。FTIR可应用于术中甲状腺癌转移性淋巴结的探测。The aim of the study is to explore the possibility of detecting metastatic lymph nodes by FTIR spectra during the surgery of thyroid cancer. The FTIR spectra of 20 metastatic lymph nodes and 69 non-metastatic ones were collected via an ATR （attenuated total reflectance） probe. For each spectrum,28 variables of 13 bands including peak positions and relative intensities were measured. The variables of metastatic lymph node were compared to those of non-metastatic ones using standard statistic methods. The results indicated that the FTIR spectra of metastatic lymph nodes were significantly different from non-metastatic ones in the bands related to protein,lipid,nucleic acid and carbohydrate. （1） Variations of bands related to protein：The relative intensity ratios of I3 280/I1 460,I1 640/I1 460 and I1 546/I1 460 increased significantly （P〈0.05）; （2） Variations of bands related to lipid：The relative intensity ratios of I1 743/I1 460 decreased significantly （P〈0.05）. On the contrary,I1 400/I1 460 increased significantly （P〈0.05）; （3） The peak positions of 1 165 and 1 120 cm^-1 which were all assigned to carbohydrate shifted toward higher wave number （P〈0.05）. The relative intensity ratio of I1 165/I1 460 decreased significantly （P〈0.05）; （4） The peak positions of 1 085 cm^-1 related to nucleic acid shifted to the lower wave number （P〈0.05）; （5） Other undetermined bands：The relative intensity ratios of I1 303/I1 460 and I1 303/I1 240 increased significantly （P〈0.05）. FTIR spectroscopy could be a reliable and practicable method for metastatic lymph nodes diagnosis in the operation of thyroid cancer. It could be applied in detecting metastatic lymph nodes which can not be determined by palpation in surgery.

关 键 词：淋巴结 甲状腺癌 FTIR光谱 

分 类 号：O657.3[理学—分析化学]