三种不同光谱学方法测定转基因抗虫棉组织营养元素含量  

Determination of Nutrient Elements in Transgenic Insect-Resistant Cotton Tissues by Three Different Spectroscopical Methods

作　　者：孙彩霞[1] 张玉兰[2] 孙玉全[1] 杨磊[1] 王杰[1] 崔振波[1] 

机构地区：[1]东北大学理学院,辽宁沈阳110004 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《光谱学与光谱分析》2009年第11期3038-3041,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(40101016);辽宁省博士启动基金项目(20041008);辽宁省科技攻关项目(2004201003)资助

摘　　要：采用火焰原子吸收、火焰原子发射光谱法及分光光度法分析了三种不同转基因抗虫棉在不同生育时期不同组织内的11种营养元素含量的变化。结果表明,与非转基因对照比较,转基因抗虫棉植株体的营养化学组成发生了一定程度的改变,这种改变与转基因抗虫棉的品种、部位及生育时期相关。转基因抗虫棉全N含量的变化最为显著,其中转Bt抗虫棉Z30增幅最高达21%,这可能与外源基因导入及杀虫蛋白的表达有关。Mg,Na和Cu含量的显著改变仅特异的发生在某个转基因抗虫棉品种的某一组织内。外源抗虫基因导入对转基因抗虫棉植株内有机C、全P、全S、K、Ca、Fe、Zn含量的扰动是微小的,从而推测,转基因抗虫棉对这些营养元素的利用和积累能力没有显著改变。In order to find out the effects of exogenous genes,such as Bt and Bt coupled with CpTI,on nutrition metabolism in transgenic plants,totally eleven types of nutrient elements in transgenic Bt （Z30） and Bt-CpTI （CCRI41 and SGK321） cotton were determined using methods of flame atomic absorption spectroscopy,flame atomic emission spectroscopy and spectrophotometry at flowering stage and boll-opening stage. The results showed that the chemical composition of plant nutrition in transgenic insect-resistant cotton differed in comparison with non-transgenic cotton counterparts related to varieties,tissues and stages. The content of total N in transgenic cotton changed most significantly. Especially,it increased by 21% for transgenic Bt cotton Z30 compared to non-transgenic cotton Z16. These changes in total N content were probably caused by both transgenes expression in transgenic cotton and other processes not studied in this experiment. The content of Mg,Na and Cu in transgenic cotton varied significantly only in some certain varieties or tissues. It was unobvious how the incorporation of transgenes impacted on the content of organic C,total P,total S,K,Ca,Fe and Zn in transgenic cotton. The authors speculated that there were no significant changes in utilization and accumulation of these nutrient elements between transgenic insect-resistant cotton and their non-transgenic cotton counterparts （Z16,CCRI23 and SY321,respectively）.

关 键 词：转基因抗虫棉 光谱学方法 营养元素 

分 类 号：O657.7[理学—分析化学]