雪旺细胞分泌VEGF浓度的实验研究  

Study of VEGF Secreting in Cultured Schwann's Cells

作　　者：邸金[1,2] 胡盛寿[1] 周建业[1] 郭光伟[2] 李君[1] 沈雅[1] 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院卫生部心血管再生医学重点实验室,100037 [2]山西医科大学第二临床医学院心胸外科,030001

出　　处：《中国分子心脏病学杂志》2009年第5期287-290,共4页Molecular Cardiology of China

基　　金：自然科学基金<复合型组织工程仿生心肌室壁瘤补片的研究>(批准号30772147)

摘　　要：目的研究体外培养的雪旺氏细胞VEGF分泌量与培养时间的关系,为今后雪旺氏细胞的应用提供实验依据。方法切取2周龄SD大鼠的坐骨神经,分离、纯化、培养雪旺细胞,第三代细胞分别于12小时,1天,3天,5天,7天,9天6个时间位点收集细胞以及细胞培养液,观察细胞形态变化,S-100鉴定雪旺细胞,同时,四氮唑溴盐(MTT)比色法测定雪旺细胞的生长增值情况;ELISA法测定细胞培养液中的VEGF浓度含量。结果雪旺细胞呈长梭形,S-100蛋白表达阳性率为90%,2～5天细胞生长迅速,7天以后生长较为缓慢,分泌的VEGF量在第5天达到最高(每个时间点均P<0.05),其后第7天,第9天缓慢降低(P>0.05)。结论第3代雪旺细胞在培养到第5-7天时达到较高的分泌浓度,这个时期的细胞在血管组织工程中有较好的应用价值。Objective To study secreting of vascular endothelial growth factor （VEGF） in cultured rat Schwann＇ s cells, and to provide evidence for the cell＇ s application in vascular tissue engineering. Method Schwann ceils were harvested from the 2-week-old SD rat sciatic nerves, separated, purified, cultured and passaged twice. The supematants of the third generation of Schwann＇ s cells, at 12h,1d, 3d, 5d, 7d, 9d were collected respectively for ELISA assay. The remained cells were checked morphologically, and identified with S-100 staining separately. Results The spindle-shaped cells overlaid in the flask, and more than 90% of the cells were positive for the s-100 staining. The VEGF level increased at 12h, ld, 3d, 5d, and then decreased at 7d, 9d respectively. Conclusions The cultured 3-5 days of the 3rd generation Schwann＇ s cells, with highest VEGF levels, were applicable in vascular tissue engineering.

关 键 词：雪旺细胞 血管内皮生长因子 ELISA法 浓度 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]