羟基红花黄色素A双罐组式逆流提取工艺研究  

Research on technological process of two-pot countercurrent extraction of hydroxysafflor yellow A

作　　者：李页瑞[1] 陈勇[1] 王龙虎[1] 陈雪英[1] 刘雪松[1] 孙长海[2] 闫文杰[2] 

机构地区：[1]浙江大学药学院,浙江杭州310058 [2]天津红日药业股份有限公司,天津301700

出　　处：《中国中药杂志》2009年第21期2743-2747,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A08)

摘　　要：目的:研究以红花药材为原料,采用罐组式逆流提取技术提取羟基红花黄色素A(HSYA)的最优工艺条件。方法:考察了阶段提取时间、提取温度、乙醇浓度、料液比(g.mL-1)等因素对提取效果的影响,选用L9(34)正交试验法对提取工艺进行优化研究,并与传统浸提法和药典所述方法进行了比较。结果:阶段提取时间和料液比两个因素对HSYA提取率有显著性影响,最佳提取工艺为用10倍药材质量的30%乙醇,在室温下每个阶段提取120 m in。在此最佳工艺条件下,HSYA提取率为1.56%,浸出物中HSYA纯度为6.06%。与传统浸提法和药典中所述的超声提取法相比,提取率分别提高6.12%和9.09%,纯度分别提高42.9%和27.0%。结论:罐组式逆流提取技术具有操作简便、溶剂用量少、提取率和纯度较高等优点,具有很大的工业化应用价值。Objective： To study the optimum technical conditions of extracting Hydroxysafflor yellow A （HSYA） from Carthmus tinctorius by multi-stage countercurrent extraction technology. Method： The effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio （ g · mL^-1 ） on extraction yield of HSYA were studied by orthogonal test design and the comparison of other extraction methods were presented. Result： Extraction time and solid-liquid ratio had significant influence on the extraction yield, and the optimum parameters were as follows： Extraction time of each stage was 120 min, solid-liquid ratio was 1：10 （g · mL^-1 ）, ethanol concentration was 30%, and extracted at room temperature. Under the optimum conditions, the extraction yield of HSYA was 1.56% and the purity of the extract was 6.06%. Compared with the traditional extraction method and the ultrasonic extraction method of the pharmacopoeia, the extraction yield was increased by 6. 12% and 9. 09% , the purity of extract was increased by 42. 9% and 27. 0%, respectively. Conclusion： The multi-stage countercurrent extraction technology has many advantages such as simple operation, less solvent consumption, higher extraction yield and purity of extract and it has wide industrial application prospect.

关 键 词：羟基红花黄色素A 罐组式逆流提取技术 红花 浸提法 超声波提取法 

分 类 号：TQ461[化学工程—制药化工]