一测多评法测定吴茱萸中吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量  

Assay of evodin,evodiamine and rutaecarpine in Fructus Evodiae by QAMS

作　　者：宋亚芳[1,2] 王智民[1] 朱晶晶[1] 闫丽华[1] 张启伟[1] 龚慕辛[1] 高慧敏[1] 冯伟红[1] 王维皓[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河南中医学院,河南郑州450008

出　　处：《中国中药杂志》2009年第21期2781-2785,共5页China Journal of Chinese Materia Medica

基　　金：2007年中医药行业科技专项(200707007;200707009);国家"十一五"支撑计划项目(2006BAI08B03);<中国药典>2010年版一部标准研究

摘　　要：目的:建立吴茱萸一测多评的含量测定方法,考察不同类型化合物之间采用相对校正因子进行一测多评含量测定的准确性和可行性。方法:以吴茱萸为研究对象,建立吴茱萸次碱与吴茱萸内酯、吴茱萸碱的相对校正因子,并用该校正因子进行吴茱萸内酯、吴茱萸碱的含量计算,实现一测多评;同时采用外标法测定药材中吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量,并比较计算值与实测值的差异。结果:11批吴茱萸药材中2种成分含量的计算值与实测值间无显著差异(RSD<5%)。结论:以外标法测定吴茱萸次碱,利用相对校正因子实现对吴茱萸内酯和吴茱萸碱测定是准确的、可行的,一测多评法可以用于特定的不同类型成分间的测定。Objective： To develop a Quantitative Assay of Multi-components by Single -marker （QAMS） for simultaneous determination of three components in Fructus Evodiae, and examine the feasibility of using the relative correction factors between the different types of compounds. Method： Rutaecarpine was selected as the internal reference substance; the relative correction factors of evodin and evodiamine were calculated. The contents of three components in 11 batches of samples were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results. Result： No obvious differences （RSD 〈 5 % ） were found in the quantitative results of evodin and evodiamine in 11 batches of Fructus Evodiae determined by the two methods. Conclusion： It is feasible and suitable to determine evodin and evodiamine in Fructus Evodiae by QAMS, and this method can be used for a certain different types of compounds.

关 键 词：“一测多评”法 吴茱萸 吴茱萸内酯 吴茱萸碱 吴茱萸次碱 

分 类 号：R284[医药卫生—中药学]