丹参酚酸B盐对大鼠肾纤维化的影响  

Effect of salvianolic acid B on generation and activation of myofibroblast in rat with renal interstitial fibrosis

作　　者：周娟[1] 张悦[1] 陆海英[2] 刘煜敏[1] 林敏[1] 

机构地区：[1]上海中医药大学科技实验中心,上海201203 [2]上海中医药大学护理学院,上海201203

出　　处：《中国中药杂志》2009年第21期2790-2793,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30500687);上海市高等学校科学技术发展基金项目(05CZ23);上海市教育委员会重点学科(第五期)(J50301)

摘　　要：目的:观察丹参酚酸B盐(SA-B)对单侧输尿管梗阻大鼠肾间质纤维化的影响。方法:将雄性SD大鼠随机分为假手术组(12只)、模型组(12只)和治疗组(12只)。模型组大鼠行单侧输尿管结扎(UUO)建立肾小管间质纤维化模型。治疗组在UUO基础上给予SA-B水溶液(12.5 mg.kg-1)灌胃,各组于造模后第14,21天分2批处死。用苏木精-伊红(HE)染色观察肾组织病理学改变,用免疫组织化学方法观察α平滑肌肌动蛋白(α-SMA)的表达,用逆转录-多聚酶链反应(RT-PCR)方法检测肾组织中角蛋白19 mRNA的表达。结果:SA-B能够显著改善梗阻侧肾脏的病理改变,显著减少肾组织α-SMA的表达,增加角蛋白的表达。结论:SA-B可明显改善UUO所致的大鼠肾间质纤维化,其作用可能是通过阻止小管上皮-成肌纤维细胞转分化和抑制成肌纤维细胞的增生和活化而实现的。Objective： To investigate the effect of salvianolic acid B （SA-B） on renal interstitial fibrosis due to unilateral ureteral obstruction. Method： Thirty-six SD male rats were randomly divided into 3 groups, 12 in each group, the sham-operated group, the model group and the SA-B treated group. The rat model of renal interstitial fibrosis was successfully established by unilateral ureteral obstruction （UUO）. Rats in the SA-B treated group was intragastrically administrated with SA-B （ 12. 5 mg · kg^-1 ） daily after modeling. Rats of each group were killed respectively at day 14 and day 21 after UUO. Pathological changes of renal tissue were observed by hematoxylin and eosin （HE） staining. The expression of a-smooth muscle actin （α-SMA） in kidney was determined with immunohistochemistry. And the expressions of cytokeratin19 （ck19） mRNA in renal tissue were detected using reverse transcription polymerase chain reaction （RT-PCR）. Result： Renal interstitial fibrosis was obviously ameliorate in SA-B treated group. The expression of α- SMA was significantly decreased in SA-B treated group as compared with that in model group at day 14. And the expression of ck19 was significantly lower than that determined in model group at day 21. Conclusion： SA-B could ameliorate renal interstial fibrosis due to UUO, probable by inhibiting epithelial-to-myofibroblast transdifferentiation and the activation of myofibroblast.

关 键 词：丹参酚酸B盐 肾间质纤维化 小管上皮-成肌纤维细胞转分化 

分 类 号：R285.5[医药卫生—中药学]