川芎嗪调控血管内皮生长因子与抗大鼠佐剂性关节炎的相关性研究  

Relationship of tetramethylpyrazine on expression of vascular endothelial growth factor and development of adjuvant-induced arthritis in rats

作　　者：马强[1] 陈刚[2] 张继芬[1] 冯彬彬[1] 徐晓玉[1] 

机构地区：[1]西南大学药学院中药学院,重庆400715 [2]重庆工商大学药物化学与化学生物学研究中心,重庆400067

出　　处：《中国中药杂志》2009年第21期2799-2802,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30070915)

摘　　要：目的:探讨川芎嗪是否可通过调节血管内皮生长因子(VEGF)表达而影响大鼠实验性关节炎的发展。方法:建立大鼠佐剂性关节炎(AIA)模型,灌胃给药川芎嗪,检测大鼠足垫厚度的变化,免疫组化SP法染色CD34检测滑膜微血管密度(MVD),放射免疫法分析大鼠血清中肿瘤坏死因子(TNF-α)的含量,Western blot分析滑膜组织VEGF的表达。结果:与模型组相比较,100 mg.kg-1川芎嗪组大鼠足垫厚度显著减小,血清中TNF-α的含量和滑膜组织VEGF的表达均明显降低。结论:川芎嗪抑制大鼠佐剂性关节炎的发展与抑制病变关节滑膜VEGF的表达密切相关。Objective： To explore the effect of tetramethylpyrazine （TMP） on the expression of vascular endothelial growth factor （VEGF） in the synovium of adjuvant-induced arthritis （AIA） in rats. Method： AIA rats were treated with different doses of TMP. The effects of treatment were monitored by footpad thickness, contents of tumor necrosis factor-α （TNF-α） in serum, microvessel density （MVD） and the expression of VEGF protein in the synovium. Result： Compared with arthritis model, 100 mg · kg^-1 TMP could remarkably reduce the footpad thickness, contents of TNF-α in serum and the expression of VEGF in the synovium. Conclusion： TMP can attenuate the degree of chronic inflammation in AIA rats, and its mechanism might be associated with inhibiting the expression of VEGF.

关 键 词：川芎嗪 血管内皮生长因子 佐剂性关节炎 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]