黄芩茎叶总黄酮对高甘油三酯血清刺激的VSMCs增殖的影响  

Effects of Scutellaria baicalensis stem-leaf total flavonoid on proliferation of vassel smooth muscle cells stimulated by high triglyceride blood serum

作　　者：罗雪磊[1] 周晓霞[1] 苏佩清[1] 朱网娣[1] 

机构地区：[1]扬州大学医学院,江苏扬州225009

出　　处：《中国中药杂志》2009年第21期2803-2807,共5页China Journal of Chinese Materia Medica

基　　金：扬州大学自然科学基金课题(PK0513124)

摘　　要：目的:观察黄芩茎叶总黄酮(SSTF)对单纯高甘油三酯血清(HTG)刺激的血管平滑肌细胞(VSMCs)增殖的抑制作用及可能的分子机制。方法:体外培养大鼠胸主动脉VSMCs,HTG诱导其增殖,观察SSTF对细胞增殖、细胞周期、CO含量、血红素氧化酶-1(HO-1)和ERK/p-ERK蛋白表达的影响。结果:500 mg.L-1的SSTF可明显抑制HTG诱导的VSMCs增殖,增加VSMCs培养上清液中CO的生成量,抑制细胞周期进程(P<0.05,P<0.01),并呈时间和剂量依赖趋势;500 mg.L-1的SSTF显著诱导细胞ERK/p-ERK蛋白的表达(P<0.01),但是对HO-1蛋白的表达无影响。结论:SSTF通过阻滞细胞周期进程直接参与抑制VSMCs增殖的作用,ERK信号通路可能参与SSTF介导的细胞保护作用。Objective： To determine the effects and related mechanism of Scutellaria baicalensis stem-leaf total flavonoid （SSTF） on vascular smooth muscle cells （VSMCs） proliferation induced by high triglyceride blood serum （HTG）. Method： VSMCs isolated from rat aorta were cultured in vitro and proliferation was stimulated by HTG, SSTF was added to influence the proliferation of VSMCs. The proliferation and cell cycle of VSMCs were determined by MTT assay and flow cytometry, respectively. CO released into the culture media was quantitated by measuring carbon monoxide hemoglobin （COHb）. The protein expressions of HO-1 and extracellular signal-regulated kinase （ERK/p-ERK） were detected by Western blot analysis. Result： 500 mg·L^-1 SSTF could obviously suppress the cell multiplication by HTG＇s induction, remarkably increase the production of COHb in VSMCs, obviously suppresse the mitotic cycle progress of VSMCs （P 〈0. 05, P 〈0.01 ） , in the time and dosage dependence. Furthermore, 500 mg · L^-1 SSTF remarkably declined the ERK/p-ERK protein expression （P 〈 0. 01 ）, but did not have the influence on the HO-1 protein＇s expression. Conclusion ： SSTF inhibited the proliferation of VSMCs directly by blocking cell cycle progression, and the ERK signal transduction way possibly participated in the cytoprotection of SSTF.

关 键 词：黄芩茎叶总黄酮 高甘油三酯血清 VSMCS HO-1 ERK 

分 类 号：R285.5[医药卫生—中药学] R543.5[医药卫生—中医学]