人参皂苷Rb1对白消安干预人脐静脉内皮细胞一氧化氮合成及转化生长因子-β1表达的影响  

Effects of ginsenoside Rb1 on busulfan-induced variation of nitrogen synthesis and TGF-β1 expression in human umbilical vein endothelial cells

作　　者：陈红霞[1,2] 谢薇[1] 魏文宁[1] 夏凌辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液内科,武汉430022 [2]咸宁学院基础医学院机能系,湖北咸宁437100

出　　处：《临床血液学杂志》2009年第6期603-605,共3页Journal of Clinical Hematology

摘　　要：目的:研究白消安(BU)对人脐静脉内皮细胞(HUVEC)一氧化氮(NO)合成及转化生长因子-β1(TGF-β1)表达的干预,并进一步探讨人参皂苷Rb1对此干预作用的影响。方法:体外培养HUVEC,以硝酸还原酶法检测各组细胞培养上清液中NO的含量,ELISA方法检测上清液TGF-β1蛋白水平,实时荧光定量PCR(Real Time-PCR)法检测HUVEC中内皮源性一氧化氮合酶(eNOS)mRNA表达。结果:BU明显抑制HUVEC合成NO及eNOSmRNA表达,而刺激TGF-β1的表达;预先用人参皂苷Rb1干预再加BU作用,发现人参皂苷Rb1可以拮抗BU对HUVEC的这种影响,提高eNOSmRNA表达及NO合成,而降低TGF-β1的表达。结论:BU可抑制HUVEC合成NO而增强TGF-β1表达,人参皂苷Rb1可拮抗此作用,人参皂苷Rb1可能籍此机制保护血管内皮细胞免受化疗药物损伤。Objective：To investigate the effects of ginsenoside Rb1 on busulfan（BU）-induced variation of nitrogen oxide（NO） synthesis and TGF-β1 expression in human umbilical vein endothelial cells（HUVECs）.Method：HUVECs were cultivated in vitro.NO level in cell supernatant was tested by nitrate reductase assay.Protein level of TGF-β1 was tested by ELISA.Endothelial endothelial nitric oxide synthase（eNOS） mRNA expression was detected by real-time PCR.Result：Level of NO and expression of eNOS mRNA in HUVEC were significantly inhibited by BU,while the protein level of TGF-β1 was enhanced by BU.Ginsenoside Rb1 reversed these effects of BU in HUVEC,thus resulted in increased synthesis of NO and decreased expression of TGF-β1.Conclusion：BU could inhibit synthesis of NO and TGF-β1 in HUVEC,while ginsenoside Rb1 could reverse these effects.Ginsenoside Rb1 might protect human vascular endothelium from chemotherapeutic reagent-induced damage through this mechanism.

关 键 词：人参皂苷RB1 内皮源性一氧化氮合酶 一氧化氮 

分 类 号：R284[医药卫生—中药学]