铁皮枫斛颗粒对辐射诱导的小鼠脾脏淋巴细胞凋亡影响的实验研究  

Study on Preventive and Therapeutic Effect of Dendrobium Candidum wall.ex Lindl on Rat with Splenocytes Injury Induced by Radiation Exposure

作　　者：杨春桥[1] 张国庆[1] 刘玉龙[1] 顾柏林[1] 秦秋荣[1] 孙静平[1] 

机构地区：[1]苏州大学附属第二医院中医科,江苏苏州215004

出　　处：《中国血液流变学杂志》2009年第3期359-361,共3页Chinese Journal of Hemorheology

摘　　要：目的探讨中药铁皮枫斛颗粒（DCWD）对辐射诱导的BALB／C小鼠脾淋巴细胞凋亡的影响。方法清洁级雄性BALB／C小鼠，随机分成对照组、模型组、DCWD低剂量组和DCWD高剂最组。用4Gy的6MV-X射线全身单次均匀照射小鼠，照射后6h处死小鼠，用流式细胞仪检测脾淋巴细胞凋亡情况，并用免疫组化染色试剂盒检测其Bcl-2和Bax的表达水平。结果DCWD组细胞凋亡、细胞凋亡率及Bax表达率则低于模型组，而Bcl-2表达率高于模型组，且DCWD低剂量组和高剂量组组间差异无统计学意义（P〉0．05）。结论DCDW可抑制辐射诱发的小鼠脾淋巴细胞凋亡及Bcl-2和Bax蛋白表达异常，对辐射诱导小鼠脾淋巴细胞损伤可能具有保护作用。Objective To investigate the effect of Dendrobium candidum wall.ex LindI（DCWD） on the cell apoptosis and expressions of Bcl-2 and Bax protein in splenocytes of mice irradiated with gamma rays.Method Clean male BALB/c mice were randomly divided into control group, modol group,DCWD low-dose and DCWD high dose group.DCWD was intragastrically administrated to mice and then irradiated with X ray at one time.All mice was executed on the six hours after the irradiation.Spleen lymphocyte apoptosis and apoptosis rate by FCM and immunohistochemistry detection kit Bcl-2 and bax expression.Results Splenocytes apoptosis and its rate and the Bax expression of the DCWD protection group was lower than that in the irradiated control group. But the Bcl-2 expression was higher than that in the irradiated control group.And lower protection group was not different from higher protection group.Conclusion The present study showed that DCWD can inhibit radiation induced cell apoptosis and abnormal expression of Bcl-2 and Bax protein in splenocytes, and can prevent acute radiation injury.

关 键 词：铁皮枫斛颗粒 放射损伤 脾淋巴细胞 凋亡 BCL-2 BAX 

分 类 号：R818.74[医药卫生—放射医学] R285.5[医药卫生—临床医学]