链霉菌FR-008大线性质粒pHZ227末端的克隆  

Cloning telomeres of large linear plasmid pHZ227 in Streptomyces sp. FR-008

作　　者：肖湘[1] 周秀芬[2] 邓子新[2] 

机构地区：[1]国家海洋局第三海洋研究所,厦门361005 [2]华中农业大学生命科技学院,武汉430070

出　　处：《台湾海峡》1998年第4期448-450,共3页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金!39430010

摘　　要：采用中性溶菌结合超速离心的方法，从链霉菌FR－008中直接提取了完整的约130kb的大线性质粒pHZ227，经蛋白酶K和限制性内切酶处理后，进行强迫克隆。限制性内切酶酶解和杂交实验证实已克隆到大线性质粒pHZ227的末端。The DNA of Streptomyces sp. FR-008 large linear plasmid pHZ227 was isolated by ultracentrifugation. The isolated DNA was purified by proteinase K and digested with PstI. ThepBluscript M13(-) vector DNA was digested with PstI and Smal. E. coli DH5 a was transformed with the ligated DNA. The ampicillin resistant transformants were isolated. Transformants containing .telomeres were purified and confirmed by restriction digestion and hybridyzation.

关 键 词：链霉菌 线性质粒 蛋白酶K 强迫克隆 

分 类 号：Q939.1[生物学—微生物学] Q731