抗真菌药敏试验ATBFUNGUS2微量稀释法、ROSCO纸片法和NCCLS纸片法的对比研究  

Compartive study for ATBFUNGUS2 microdilution,ROSCO disk diffusion and NCCLS disk diffusion on antifungal susceptibility testing

作　　者：陈霁明[1,2] 姚涌[1] 汪学龙[1] 

机构地区：[1]安徽省医科大学病原生物学教研室,合肥230032 [2]安徽省池州市第二人民医院检验科

出　　处：《热带病与寄生虫学》2008年第1期5-7,共3页Journal of Tropical Diseases and Parasitology

摘　　要：目的对比研究白色假丝酵母菌的体外抗真菌药敏试验ATBFUNGUS2微量稀释法、ROSCO纸片法和NCCLS纸片法。方法采用前述3种方法,检测105株白色假丝酵母样真菌对氟康唑、依曲康唑、两性霉素、氟胞嘧啶的敏感性。结果体外抗真菌药敏试验ATBFUNGUS2微量稀释法、ROSCO纸片法和NCCLS纸片法用于检测白色假丝酵母样真菌有较好的一致性、重复性。结论ATBFUNGUS2微量稀释法和ROSCO纸片法可以作为较好的体外抗真菌药敏试验方法在临床实验室推广使用。Objective To compare the methods ofATBFUNGUS2 microdilution, the R.OSCO disk diffusion and the NCCLS disk diffusion in ex＇tracorporeal antifungal susceptibility testing. Methods A total of 105 clinical fungal isolates were tested for susceptibility to fluconazole, itraconazole, AMB, 5-FC by the three methods respectively. Results The results of the antifungal susceptibility testing of the three methods had fine consistency and repeatability. Conclusion The methods of ATBFUNGUS2 microdilution and the Rosco disk diffusion can be used as extracorporeal antifungal susceptibility testing in clinic laboratories.

关 键 词：药敏试验 NCCLS纸片法 微量法 ROSCO纸片法 真菌 对比研究 

分 类 号：R978.5[医药卫生—药品] R446.1[医药卫生—药学]