胶体金免疫层析法在布鲁氏菌病监测地区的现场应用  

Field application of gold immunchromatography assay in brucellosis surveillance area

作　　者：邵丰尧[1] 朱匡纪[1] 朱明东[2] 

机构地区：[1]浙江省金华市婺城区疾病预防控制中心,浙江金华321000 [2]浙江省医学科学院

出　　处：《疾病监测》2009年第10期753-754,共2页Disease Surveillance

基　　金：浙江省科学技术厅基金资助(No.2006F13022)

摘　　要：目的验证胶体金免疫层析法(GICA)在布鲁氏菌病监测地区的现场应用效果。方法选择布鲁氏菌病监测地区重点人群和布鲁氏菌病流行奶牛场的牛,采用GICA和虎红平板凝集试验(RBPT)进行检测。结果监测地区重点人群检测结果GICA和RBPT两种方法阳性率分别为15.3%和11.4%;两种方法检测结果差异无统计学意义(P>0.05)。奶牛监测结果,GICA和RBPT两种方法阳性率分别为14.4%和16.0%;两种方法检测结果差异无统计学意义(P>0.05)。结论GICA与RBPT同样具有较好的敏感性;此外,GICA还具有操作简便、快速、微量等优点,适合于布鲁氏菌病监测地区的现场应用。Objective To evaluate the field application of gold immunchromatography assay （GICA） in brucellosis surveillance area. Methods GICA and rose bengal precipitation test （RBPT） were used to conduct parallel detection of sera from the persons who were at risk of brucellosis and in surveillance area and the cows in dairy farm where brucellosis had occurred. Results The positive rates of two methods for human were 15.3% and 11.4% respectively, the difference had no statistical significance （ P 〉 0.05 ）, and the positive rates of two methods for cow were 14.4% and 16.0% respectively, the difference had no statistical significance （P 〉 0.05 ）. Conclusion ,Both GICA and RBPT are sensitive, but GICA is simple, rapid and requires small amount of sample, which is suitable for the field application in brucellosis surveillance area.

关 键 词：胶体金免疫层析法 布鲁氏菌病 监测地区 现场应用 

分 类 号：S855[农业科学—临床兽医学]