不可分群脑膜炎奈瑟菌PCR基因分型研究  

PCR genotyping of ungroupable Neisseria meningitides

作　　者：张艳亭[1] 徐丽[2] 高源[2] 杨慧芳[1] 刘宝莲[1] 芦晶[1] 李海清[1] 

机构地区：[1]山西省阳泉市疾病预防控制中心,山西阳泉045000 [2]中国疾病预防控制中心传染病预防控制所

出　　处：《疾病监测》2009年第10期779-781,共3页Disease Surveillance

摘　　要：目的对自健康人群鼻咽拭子中分离的74株表型特征为不可分群的脑膜炎奈瑟菌,利用PCR基因分型技术进行基因特征分析。方法用PCR方法扩增脑膜炎奈瑟菌种属特异性基因crgA和A、B、C、W135、Y、Z、X以及29E不同群的特异性基因片段,确定不同菌株的基因型。结果以脑膜炎奈瑟菌crgA基因为靶基因进行PCR扩增,74株不可分群脑膜炎奈瑟菌中,66株脑膜炎奈瑟菌crgA基因扩增阳性,检出率89.19%。66株菌株进行不同血清群的PCR分群鉴定,40株菌株(60.61%)可检测为不同的血清群基因型,B群27株,29E群7株,X、Y群各2株,C、W135各1株。结论不可分群脑膜炎奈瑟菌进行PCR基因分型研究对脑膜炎奈瑟菌的研究具有重要的流行病学意义。Objective PCR genotyping analysis of the genetic characteristics of 74 phenotypically ungroupable Neisseria meningitidis derived from the nose and throat swabs among healthy population was conducted. Methods PCR amplification of Neisseria meningitidis genus-specific genes, crgA and A, B, C, W135, Y, Z, X, as well as 29E of specific gene fragments of different groups, was performed to identify the genotypes of different swains. Results PCR amplification based on Neisseria meningitidis crgA as the target gene revealed 66 crgA-positive Neisseria meningitidis out of 74 ungroupable strains, the detection rate being 89. 19%. PCR serogroup identification suggested 40 strains （60.61%） of different serogroups of genotypes among the 66 strains, including 27 strains of B group, seven of 29E group, two of X and Y each, and one of C and W135 each. Conclusion PCR genotyping analsys of ungroupable Neisseria meningitidis exerted a significant epidemiologic impact on the study of Neisseria meningitidis.

关 键 词：不可分群 脑膜炎奈瑟菌 PCR 

分 类 号：R515.2[医药卫生—内科学]