东方田鼠肝脏cDNA文库的构建及部分克隆序列分析  

Construction and sequence analysis of a cDNA library from the liver of Microtus fortis

作　　者：冯洁[1,2,3] 谢建云 孙强[4] 杨玉琴[5] 胡建华 高诚 

机构地区：[1]上海市实验动物质量监督检验站,上海201203 [2]上海市计划生育科学研究所,上海200032 [3]上海实验动物研究中心,上海200032 [4]华东师范大学脑功能基因组学研究所,上海200062 [5]复旦大学上海医学院,上海200032

出　　处：《兽类学报》2009年第4期447-450,共4页Acta Theriologica Sinica

基　　金：上海市科学基金资助项目(071409001)

摘　　要：The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu /μL,the recombination rate was about 94%,the library capacity was 1.08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.The plasmid cDNA library from the liver of Microtusfortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu/μL, the recombination rate was about 94% , the library capacity was 1.08 × 10^6. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences, it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice, rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology, fatty liver model development and pathogenesis research of Microtus fortis.

关 键 词：CDNA文库 东方田鼠 肝脏 

分 类 号：Q7[生物学—分子生物学]