猪伪狂犬病病毒gD基因的生物信息学分析  

Bioinformatics analysis of pseudorabies virus gD gene

作　　者：徐志文[1] 郭万柱[1] 许雁峰[1] 朱玲[1] 张博[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014

出　　处：《中国兽医学报》2009年第11期1382-1389,1394,共9页Chinese Journal of Veterinary Science

基　　金：四川省科技厅基础项目(03JY029-40)

摘　　要：自测12条PRV不同毒株的gD全序列连同GeneBank中登录的9条gD基N全序列共21条基因序列，使用生物软件对它们的基因序列的同源性、突变区域的定位、遗传进化关系、酶切位点的差异、密码子偏爱性，氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、三级结构预测等生物信息学的内容进行预测和分析。结果表明：PRV-gD基因的开放阅读框的核苷酸长度在1197-1215nt之间，氨基酸长度在399-405个之间，核酸同源性在97.3％-100％之间，氨基酸的同源性在89.8％-98.8％之间，在核酸820-837位有个高变重复区，不同毒株基因均对GC极为偏爱。在遗传进化关系上将我国PRV流行分为四川、华北、东南3个区域。毒株间基因内酶切位点、蛋白质亲水性、抗原表位和蛋白质高级结构预测等内容的分析结果十分相似，说明PRV-gD基因具有很高的保守性。Twelve sequenced gD genes of different PRV strains and 9 gD genes from GenBank were compared by bioinformatics software to analyze and predict nucleotide homology,location of mutation area, phylogenetic tree, enzyme site,codons bias,amino acids homology,protein hydrophilicity,epitope and three-dimensional structure. The resuits showed that the ORF length of PRV-gD gene is 1197 - 1215 nt, amion acids length is 399-405, nucleotide homology is 97.3%- 100% ,amino acids homology is 89.9%-98.8% ,a hypermutation replicated plot is located in 820 -837 nt and different strains were prefer to GC hasi-. According to the phylogenetie tree, prevalence of PRV in China can be divided into three regions,Sichuan province, Northern China and Southeast China. The enzyme site, protein hydrophilicity,epitope and three-dimensional structure prediction of different strains were very similar, suggesting that the gD gene of PRV is highly conserved.

关 键 词：伪狂犬病病毒 GD基因 PCR 序列分析 生物信息学 

分 类 号：S852.65[农业科学—基础兽医学]