雌激素受体α在雌性大鼠脑中的表达  

Expression of estrogen receptor α in female rat's brain

作　　者：吴建云[1] 田茂春[1] 王鲜忠[1] 范光丽[2] 张家骅[1] 

机构地区：[1]西南大学动物科技学院重庆市牧草与草食家畜重点实验室,重庆北碚400715 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国兽医学报》2009年第11期1489-1494,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30700587);西南大学博士启动基金项目

摘　　要：本试验利用免疫组织化学SP法,研究了雌激素受体α在大鼠脑中的表达。结果表明,ERα免疫反应产物主要定位于神经元细胞核,部分区域出现阳性神经元和阳性突起,在海马CA1、CA2辐射层、齿状回、下托的分子层等出现阳性星形胶质细胞;ERα在脑中表达广泛,免疫反应产物在端脑的隔外侧核、海马锥体细胞层、大脑皮质等,间脑的缰内侧核、下丘脑室旁核、弓状核、视上核等,中脑的动眼神经核,脑桥的脑桥核外侧部等为高密度;在端脑的尾壳核、杏仁中央核、海马CA4区,间脑的下丘脑室周核、缰外侧核等,中脑的黑质致密带,脑桥的脑桥被盖核等为较高密度;在间脑的视前室周核、丘脑后核、中脑的红核、脑桥的脑桥被盖网状核等为中等密度;在端脑的苍白球,间脑的无名质,无免疫反应产物出现。结果显示,雌激素在调节脑的认知和生殖内分泌过程中,ERα可能起主要作用;雌激素可通过ERα调节星形胶质细胞功能。The expression of ERo was systematically studied in rat＇s brain by immunohistochemistry SP method. The results were as follows： ERa-IR products were mainly located in the karyons of neurons and there were positive neurons or neurites in the part of regions and existed positive astrocytes in hippocamp CA1,CA2 lamina radiatum, molecular lamina in gyrus fasciolaris and subiculum,et al. ERo expressed widely in the brain. The density of ERa-IR products were high in nucleus septi lateralis, hippocampus lamina pyramidal cell, the cortex; et al in telencephalon, nucleus medialis habenulae, nucleus paraventricularis hypothalami, nucleus arcuatus, nucleus supra-opticus; et al in diencephalon, nucleus nervi oculomotorii in mesencephalon, pars media/is nucleus pontis;et al in pontis. ERa-IR prod- ucts were higher in nucleus caudatus putamen,nucleus amygdaloieus medialis and hippocamp CA4;in telencephalon, nucleus periventricularis hypothalami, nucleus lateralis habenulae; et al in diencephalon, substantia nigra in mesen-cephalon and nucleus tegmenti pontis;et al in pontis. ERo-IR products were medium in nucleus pre-opticus periven- tricularis, nucleus posterior thalami in diencephalon,nucleus tuber in mesencephalon and nucleus reticularis tegmenti pontis in pontis. There were not ERa-IR products in globus paltidus and substantia innominata. The above results reveled that ERa might play a vital role in the cognition and reproductive endocrinology process of brain modulated by estrogen and estrogen could regulate the function of astrocytes by ER.

关 键 词：大鼠 雌激素受体Α 免疫组化 

分 类 号：S831.1[农业科学—畜牧学]