福氏2a志贺菌DNA转录激活蛋白MarA的二级结构及其B细胞抗原表位预测  

Prediction of the secondary structure and B cell epitopes for the DNA-binding transcriptional activator protein MarA of Shigella flexneri 2a

作　　者：魏小娟[1] 张继瑜[1] 王松泰[1] 李剑勇[1] 周旭正[1] 牛建荣[1] 李金善[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院新兽药重点开放实验室,甘肃兰州730050

出　　处：《黑龙江畜牧兽医》2009年第11期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30671582)

摘　　要：研究以福氏2a志贺菌(Shigella flexneri2a,s.f2a)的基因组序列为材料,采用Gamier-Robson法、Chou-Fasman法和Kamplus-Schulz法预测了其DNA结合转录激活蛋白MarA的二级结构,用Kyte-Doolittle法对结构蛋白的亲水性进行了分析,用Emini法预测了蛋白的表面可能性,以Jameson-Wolf法预测了蛋白的抗原指数,然后综合评价了福氏2a志贺菌转录激活蛋白MarA的B细胞抗原表位。结果表明:福氏2a志贺菌MarA蛋白的二级结构较为复杂,含有较多的转角和无规则卷曲等柔性区域以及α-螺旋和β-折叠区段,该蛋白有多处抗原指数较高的区段,其中含有潜在的B细胞优势抗原表位,因此其在免疫学中的地位也应当引起重视。To predict the secondary structure and B cell epitopes for DNA - binding transcriptional activator MarA of Shigella flexneri 2a , the secondary structure of DNA - binding transcriptional activator protein MarA was predicted by the methods of Gamier - Robson, Chou - Fasman and Kamplus -Schulz based on Shigellaflexneri 2a genome,and hydropbilicity. Surface probility plot and antigenic index were obtained by the methods of Kyte - Doolittle, Emini and Jameson - wolf respectively, Combining the results according to these methods, the B cell epitopes for DNA - binding transcriptional activator protein MarA of Shigellaflexneri 2a were predicted. The results showed that there are much flexible region such as coil region and turn region in DNA - binding transcriptional activator protein MarA of Shigellaflexneri 2a,and there are more predominant B cell epitope. This study would be helpful for identification of B cell epitopes for protein using experimental methods and research of revere vaccine of Shigella flexneri 2a.

关 键 词：福氏2A志贺菌 DNA结合转录激活蛋白MarA 二级结构 B细胞表位 

分 类 号：S852.6[农业科学—基础兽医学]