海洋细菌Bacillus subtilis次级代谢产物的研究  

Studies on the secondary metabolites from the marine bacteria Bacillus subtilis

作　　者：刘涛[1] 李占林[1] 王宇[1] 王业玲[2] 田黎[3,4] 裴月湖[1] 华会明[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]中央民族大学生命与环境科学学院,北京100081 [3]国家海洋局第一海洋研究所,山东青岛266061 [4]青岛科技大学,山东青岛26604

出　　处：《中国海洋药物》2009年第5期1-6,共6页Chinese Journal of Marine Drugs

基　　金：国家863项目(2007AA09Z413;2007AA09Z435);国家自然科学基金(20872094)

摘　　要：目的研究海洋细菌Bacillus subtilis发酵液的化学成分。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及中低压制备柱色谱进行分离纯化,通过波谱方法进行结构鉴定。结果从发酵液中分离得到11个化合物,结构鉴定为3-苄基-6-异丙基哌嗪-2,5-二酮(Ⅰ)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(Ⅱ)、苯丙酸(Ⅲ)、3-异丁基吡咯并哌嗪-2,5-二酮(Ⅳ)、3-苄基吡咯并哌嗪-2,5-二酮(Ⅴ)、胸腺嘧啶(Ⅵ)、3-苄基哌嗪-2,5-二酮(Ⅶ)、尿嘧啶(Ⅷ)、二氢胸腺嘧啶(Ⅸ)、3-甲基-8-羟基吡咯并哌嗪-2,5-二酮(Ⅹ)、3-羟甲基吡咯并哌嗪-2,5-二酮(Ⅺ)。结论化合物Ⅰ,Ⅸ,Ⅹ和Ⅺ为首次从该属细菌中分离得到。Objective To study the chemical constituents from the fermentation of marine bacteria Bacillus subtilis. Methods The compounds were separated by silica gel, Sephadex LH-20 column chromatography and MPLC, and their structures were elucidated by means of spectral analyses. Results Eleven compounds were isolated from the fermentation. Their structures were identified as 3-benzyl-6-isopropylpiperazine-2, 5-dione （ Ⅰ ）, 3-isobutyl-8-hydroxy pyrrolopiperazine-2, 5-dione （ Ⅱ ）, hydrocinnamic acid （ Ⅲ）, 3-isobutylpyrrolopiperazine-2, 5-dione （ Ⅳ ）, 3-benzylpyrrolopiperazine-2, 5-dione （ Ⅴ） ; thymine （ Ⅵ ）, 3-benzylpiperazine- 2, 5-dione （ Ⅶ ）, uracil （ Ⅷ）, dihydrothymine （Ⅸ）, 3 methyl 8-hydroxypyrrolopiperazine-2, 5-dione （ Ⅹ ）, 3 hydroxymethylpyrrolopiperazine-2, 5-dione （ Ⅺ ）. Conclusion Compounds Ⅰ , Ⅸ, Ⅹ and Ⅺ were isolated for the first time from this genus of marine bacteria.

关 键 词：海洋细菌 枯草芽孢杆菌 次级代谢产物 环二肽 

分 类 号：Q936[生物学—微生物学]