物理与化学诱变筛选产石杉碱甲高产菌株的研究  

Selection of Huperzine A High-producing Strain by Physical and Chemical Mutation

作　　者：周树良[1] 温拥军[2] 徐宁[1] 龚晓[1] 洪亚辉[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南化工职业技术学院,湖南株洲412004

出　　处：《湖南农业科学》2009年第10期1-3,8,共4页Hunan Agricultural Sciences

摘　　要：以千层塔内生真菌SHB为出发菌株,采用微波、紫外线、Co60射线、亚硝酸和硫酸二乙酯对内生真菌孢子进行诱变。诱变孢子悬液通过在PDA平板继代培养2次,把单菌落孢子转接至PD培养液中进行发酵培养,利用高效液相色谱检测发酵液,筛选出一株遗传稳定,产石杉碱甲能力较高的高产菌株wB3。其发酵产物中石杉碱甲为原始菌株的3.04倍。The spores of endophytic fungi SHB of Hupezia serrata were mutated by microwave, ultraviolet ray, Co60 ray, nitrous acid and diethyl sulfate. Then, the suspension of mutated spores was sub-cultured twice in PDA plate, and the single colony spores were transplanted to PD medium for fermentation. Finally, a Huperzine A high-producing strain -- wB3 with stable inheritance was selected by high performance liquid chromatography （HPLC）. The content of Huperzine A in fermenting product of wB3 is 3.04 times more than the primitive fungi SHB＇s.

关 键 词：内生真菌 诱变 石杉碱甲 

分 类 号：Q939.9[生物学—微生物学]