八棱海棠中转录因子基因MrDREBA6的克隆及表达分析  

Cloning and expression of transcription factor gene MrDREBA6 from Malus micromalus

作　　者：付晓燕[1] 彭日荷[1] 章镇[2] 乔玉山[2] 周军[2] 朱波[1] 高峰[1] 田永生[1] 赵伟[1] 熊爱生[1] 姚泉洪[1] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]南京农业大学园艺学院,南京210095

出　　处：《果树学报》2009年第6期761-768,共8页Journal of Fruit Science

基　　金：国家863项目(2006AA10Z117);上海市青年科技启明星跟踪计划(08QH14021);国家和上海市自然科学基金(30670179;07ZR14094;08ZR1417200);国家转基因重大专项(2009ZX08002-011B)

摘　　要：DREB类基因是植物中重要的一类转录因子基因,其编码的产物广泛参与生长发育和各类生理过程。根据拟南芥和水稻DREB类基因序列,设计2个兼并引物,利用RACE法从八棱海棠cDNA文库中扩增出一编码DREB类转录因子的cDNA序列,命名为MrDREBA6。从cDNA序列、推测的氨基酸序列、进化树、功能域和分子结构模型进行预测和较为全面的分析,表明MrDREBA6属于DREB类转录因子的A6亚族,蛋白质三级结构与AtRAP2.4和AtERF1相似。荧光定量PCR分析MrDREBA6在各种处理下表达水平显示,MrDREBA6受到PEG、ABA、高盐和低温的诱导表达。另外,组织表达特异性检测表明,正常生长条件下MrDREBA6基因在叶中表达较高,在根和茎中的表达量略低。MrDREB gene is an important family of transcription factors in plant,encoding transcriptional regulators with a variety of functions involved in the developmental and physiological processes.Here,using two degenerate primers according to the sequences of DREB genes from Arabidopsis and rice,a cDNA encoding putative DREB protein was cloned from cDNA of Malus micromalus Makino by RACE,named as MrDREBA6.Then,cDNA and deduced amino acid sequence,phylogenetic tree,function domain and three-dimension structure were predicted and analyzed.MrDREBA6 from Malus micromalus Makino was classified into A6 subgroup of DREB family transcription factor.MrDREBA6,AtRAP2.4 and AtERF1 have similar three-dimension structure.The results of Real-Time-Quantitative PCR analysis for Malus micromalus Makino exposed to various treatments demonstrated that MrDREBA6 gene was induced by PEG,ABA,high-salinity and low-temperature stresses.Expression profiling analyses of MrDREBA6 in different organs indicated that MrDREBA6 gene was mainly expressed in leaf,and faintly expressed in root and stem under normal growth condition.

关 键 词：八棱海棠 转录因子 RACE DREB类 荧光定量PCR 

分 类 号：S661.19[农业科学—果树学]