银杏铁型超氧化物歧化酶基因(GbFeSOD)的克隆与表达  

Molecular cloning,characterization and expression of iron superoxide dismutase gene from Ginkgo biloba

作　　者：李琳玲[1,2] 程华[2,3] 许锋[3] 王燕[3] 常杰[3] 程水源[2] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]黄冈师范学院生命科学与工程学院,湖北黄冈438000 [3]长江大学园艺园林学院,湖北荆州434024

出　　处：《果树学报》2009年第6期840-846,共7页Journal of Fruit Science

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0746);湖北省教育厅重大科技项目(Z200627002);湖北省青年杰出人才基金(2003AB014)

摘　　要：利用RACE技术从银杏中克隆到叶绿体铁型超氧化物歧化酶(GbFeSOD)基因cDNA全长。GbFeSOD的cDNA全长1106bp(GenBank:FJ555019),含有一个720bp的最大读码框。三维结构预测显示,GbFeSOD含有11个α螺旋和3个β折叠构成的篮子状活性中心。GbFeSOD氨基酸序列与其他植物的FeSOD具有很高的相似性。进化树分析表明GbFeSOD和其他物种起源相同。Southern杂交显示,GbFeSOD属于一个小的多基因家族。Northern杂交表明GbFeSOD在银杏的茎、叶中的表达量最高,果中表达量最小,在根中没有表达。ABA、渗透物质处理、低温和高温均能诱导GbFeSOD表达量增加,其中4℃低温诱导增加缓慢,40℃高温诱导迅速而稳定,而44℃培养条件下,GbFeSOD表达量呈先增后降的现象。The full-length cDNA sequence of Ginkgo biloba L.iron superoxide dismutase gene（GenBank：FJ555019） was isolated from Ginkgo biloba.The full-length cDNA of GbFeSOD contained a 720 bp open reading frame（ORF）.The 3D structure modeling showed that GbFeSOD contained 11 helixes and 3 sheets and had a basketry motif in the enzyme core.Phylogenetic tree analysis revealed that GbFeSOD shared the same ancestor with other FeSOD.The result of Southern analysis showed that Gb FeSOD gene was encoded with a small gene family in Ginkgo biloba.Northern hybridization analysis showed that GbFeSOD had the highest expression in leaves,then in stem,and the least in fruit.There was no expression found in roots.The expression of GbFeSOD could be induced by ABA,osmotic stress,low temperature and heat injury;among them,levels of FeSOD transcripts increased placidly a few hours after chilling stress at 4 ℃.Expression of the FeSOD gene was enhanced dramatically by heat stress at 40 ℃.However,the expression of GbFeSOD first increased and then decreased after heat stress at 44 ℃.

关 键 词：银杏 FeSOD基因 表达分析 

分 类 号：S664.3[农业科学—果树学]