逆转座子分子标记及其在果树上的应用  

Retrotransposon-based molecular markers and their applifications in fruit trees

作　　者：杜晓云[1,2] 张青林[1] 罗正荣[1] 

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070 [2]江西省农业科学院园艺研究所,江西南昌330200

出　　处：《果树学报》2009年第6期865-870,共6页Journal of Fruit Science

基　　金：国家自然科学基金(No.30871686);华中农业大学科研启动基金(No.20070504011)

摘　　要：基于逆转座子特殊的转座机制及其生物学特性发展的逆转座子分子标记,具有多态性高、基因组覆盖度广、适于高通量分析等特点。就常见逆转座子分子标记的原理、操作及其在果树种质鉴定、遗传多样性检测、亲缘关系重建和遗传图谱构建等方面的应用进展进行简要阐述,以期为此类技术在果树上的应用提供参考。Retrotransposon molecular markers developed based on the special characteristics of transposition and biology of retrotransposons are high polymorphic,dispersed almost throughout the length of all host chromosomes,capable for tracing frequency and history of retrotransposition,and suitable for automation.This paper summarizes the principles and procedures of several widely-used retrotransposon-based molecular markers and their applications in fruit trees,including germplasm identification,genetic diversity assessment,genetic relationship reconstruction,and genetic linkage mapping.This paper will be helpful for the future application of retrotransposon-based molecular techniques in fruit trees.

关 键 词：果树 IRAP REMAP SSAP RBIP 品种鉴定 遗传多样性 亲缘关系 遗传图谱 

分 类 号：S66[农业科学—果树学]