毛细管电泳-电致化学发光法测定克林霉素胶囊及人尿中克林霉素  

Determination of Clindamycin in Its Capsules and in Human Urine by Capillary Electrophoresis-Electrochemiluminescence

作　　者：彭志兵[1] 喻华娟 

机构地区：[1]宜春学院美容医学系,宜春336000 [2]江西省建筑材料工业科学研究设计院,南昌330001

出　　处：《理化检验（化学分册）》2009年第11期1285-1288,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：基于克林霉素增强联吡啶钌的电致化学发光信号,提出了一种分离检测克林霉素胶囊及人尿中克林霉素的毛细管电泳-电致化学发光方法。系统地考察了毛细管电泳工作及电致化学发光反应的条件,确定了试验条件为:①联吡啶钌浓度为5 mmol·L^(-1);②检测电位为1.15 V;③磷酸盐缓冲溶液浓度50 mmol·L^(-1);④pH值为7.5;⑤进样电压10 kV;⑥进样时间10 s。在优化试验条件下,克林霉素胶囊及人尿中克林霉素在4 min内可实现分离检测,其线性范围为5.0×10^(-7)～1.0×10^(-4)mol·L^(-1),检出限(3S/N)为1.4×10^(-7)mol·L^(-1)。Based on the enhancing effect on the electrochemil bipyridyl） ruthenium [abbreviated as Ru（bpy）3^2＋ ] by clindamycin, （ECL） of the complex tris-（2,2＇- and by combining the capillary electrophoresis （CE）, an efficient method of separation, with the ECL, a highly sensitive method of detection, contents of clindamycin in its capsules and in samples of human urine were determined effectively by CE-ECL. Through systematic study on the conditions of separation by CE and on the ECL reaction, optimum conditions were found as follow： O concn, of Ru（bpy）3^2＋ ： 5 mmol · L^-1 ; （2） potential of detection： 1.15 V; （3） conch, of PBS in ECL cell： 50 mnol · L^-1 ; （4） pH value of PBS in detection cell： pH 7. 5; （5） voltage of sample introduction： 10 kV; （6） time of sample introduction： 10 s. Linear relationship between values of detection signals and concentration of clindamycin was obtained in the range of 5.0×10^-7 --1.0×10^-4mol ·L^-1. Detection limit （3S/N） found was 1.4×10^-7mol · L^-1. Under the optimum condition, analysis of a sample of the capsule or the human urine was completed within 4min.

关 键 词：毛细管电泳 电致化学发光 联吡啶钌 克林霉素 

分 类 号：O657.8[理学—分析化学]