来氟米特对糖尿病大鼠肾脏的保护作用  

Protective effect of leflunomide to renal tissues in diabetic rats

作　　者：陈卫东 常保超[1] 杨萍[2] 张燕[1] 刘磊[1] 

机构地区：[1]安徽省蚌埠医学院第一附属医院肾病科,233004 [2]安徽省蚌埠医学院第一附属医院检验科,233004

出　　处：《实用医学杂志》2009年第21期3580-3582,共3页The Journal of Practical Medicine

基　　金：安徽省教育厅自然科学基金资助项目(编号:2005kj290)

摘　　要：目的:探讨来氟米特对糖尿病大鼠肾脏的保护作用及其作用机制。方法:将实验动物分为正常对照组、糖尿病组和来氟米特组,来氟米特组给予来氟米特治疗性灌胃。12周后检测各组大鼠24h尿蛋白定量、血糖、血清肌酐、尿素氮、肌酐清除率、肾脏标本测肾脏重量、HE及PAS染色观察肾脏病理改变。免疫组化方法检测肾组织中单核细胞趋化蛋白-1及单核/巨噬细胞特异性抗原(CD68)的表达。结果:来氟米特可以减少糖尿病大鼠24h尿蛋白排泄率及内生肌酐清除率,使大鼠肾脏组织中MCP-1和CD68的表达降低,并改变肾脏病理结构。结论:来氟米特对糖尿病大鼠肾脏有保护作用,其机制可能通过下调糖尿病大鼠肾脏MCP-1的表达,减少糖尿病大鼠肾组织单核/巨噬细胞浸润有关。Objective To investigate the renal protective effects of leflunomide in diabetic rats and to explore its mechanism. Method Sprague-Dawlay （SD）rats were divided into control rats, diabetic rats and diabetic rats treated with leflunomide. After 12 weeks, 24 hours urine protein, blood glucose, serum creatinine, blood urea nitrogen and creatinine clearance rate were detected. The kidney hypertrohy index was measured, partial nephridial tissue were treated pathology observed. Inmmunohistochemistry was used to analyse the expression of MCP-1 and CD68. Results compared with the untreated diabetic group, urinary albumin excretion and creatinine clearance rate of leflunomied treated rats were substantially decreased. The expression of MCP-1 and CD68 in leflunomide group were much lower than diabetic untreated group. Leflunomide also improved the histological changes of diabetic rats. Conclusions Leflunomide has some renal protective effect on diabetic rats, partly through down-regulating MCP-1 expression and reducing monocyte/macrophage infitration.

关 键 词：糖尿病肾病 单核细胞趋化蛋白-1 CD68 来氟米特 

分 类 号：R587.1[医药卫生—内分泌] R979.5[医药卫生—内科学]