过氧化物酶体增殖物激活受体γ_2在妊娠期糖尿病患者腹部皮下脂肪组织中的表达  

Expression of peroxisome proliferator-activated receptor γ_2 in abdominal subcutaneous adipose tissue in patients with gestational diabetes mellitus

作　　者：朱颖[1] 吴超英[1] 孙永玉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉市430022

出　　处：《实用医学杂志》2009年第21期3592-3594,共3页The Journal of Practical Medicine

摘　　要：目的:探讨过氧化物酶体增殖物激活受体(PPAR)γ2的表达变化在妊娠期糖尿病(GDM)发病机制中的作用。方法:正常妊娠和GDM孕妇各40例,每组各有20例体质指数正常者和20例肥胖或超重者。收集腹部皮下脂肪组织,分别用RT-PCR技术和Western blot印迹法检测组织中PPARγ2 mRNA及其蛋白的表达,同时测定空腹血糖、空腹胰岛素、体质指数等临床指标。结果:与正常妊娠妇女比较,孕前体质指数匹配的GDM患者均存在明显的胰岛素抵抗,但GDM两组间的胰岛素抵抗程度差异无显著性(P>0.05);GDM患者腹部皮下脂肪组织中的PPARγ2 mRNA及其蛋白质的表达水平均显著升高,肥胖或超重GDM患者尤为显著,其差异均有显著性(P<0.05)。此外,PPARγ2 mRNA及其蛋白质的表达水平与体质指数、空腹胰岛素水平呈正相关(r值分别为0.67、0.59、0.63、0.56,均P<0.01)。结论:PPARγ2表达升高参与了胰岛素抵抗的发生,与GDM的发病关系密切;PPARγ2的表达除了与血胰岛素水平显著相关外,还会受到肥胖因素的影响。Objective To investigate the expression of peroxisome proliferator-activated receptor γ2 （PPARγ2） in patients with gestational diabetes mellitus （GDM）. Methods The abdominal subcutaneous adipose tissue of 40 normal pregnant women （20 with normal BMI and 20 with obesity） and 40 GDM patients （20 with normal BMI and 20 with obesity） were collected, in which the expressions of PPARγ2 mRNA and protein were detected by RT-PCR and Western blot, meanwhile fasting blood glucose, fasting insulin, and BMI were determined. Results Insulin resistance existed in GDM patients as compared to the BMI matched normal pregnant women, but there was no significant difference of the degree of insulin resistance between different BMI patients with GDM （P 〉 0.05）. The expressions of PPARγ2 mRNA and protein in GDM patients were significantly higher than those in normal pregnant women, especially in obesity GDM patients （P 〈 0.05 ）. Moreover, the expressions of PPARγ2 mRNA and protein were positively related to BMI or fasting insulin （r = 0.67, 0.59, 0.63, 0.56, respectively, P 〈0.01 ）. Conclusion The increase of the expression of PPARγ2 is involved in the pathogenesis of insulin resistance and GDM. Fasting insulin and obesity may play an important role in the expression of PPARγ2.

关 键 词：糖尿病 妊娠 过氧化物酶体增殖物激活受体γ2 脂肪组织 胰岛素抵抗 肥胖 

分 类 号：R714.2[医药卫生—妇产科学] R587.1[医药卫生—临床医学]