粪便中大肠埃希菌分离方法的筛选  

Screening of E.coli Isolation Methods from Feces

作　　者：蒋翰鹏[1,2] 曹宾霞[3] 杨玉敏[1,2] 

机构地区：[1]国家海洋环境监测中心,辽宁大连116023 [2]大连水产学院生命科学与技术学院,辽宁大连116023 [3]大连海事大学环境科学与工程学院,辽宁大连116026

出　　处：《微生物学杂志》2009年第5期40-43,共4页Journal of Microbiology

基　　金：国家高技术研究发展计划项目(863计划;SQ2007AA09Z134008)

摘　　要：比较了滤膜法、涂布法和纸片法对粪便中大肠埃希菌的分离效果。通过对分离粪便大肠埃希菌的微量可知,纸片法与m-TEC培养基上滤膜法分离的大肠埃希菌数量结果基本一致。m-TEC培养基滤膜法分离的大肠埃希菌平均数量分别是伊红美蓝培养基涂布法分离大肠埃希菌平均数量的1.4倍、伊红美蓝培养基滤膜法分离大肠埃希菌平均数量的2.8倍、m-TEC培养基涂布法分离大肠埃希菌平均数量的2.25倍。分离粪便样品中大肠埃希菌选择滤膜法用m-TEC培养基进行分离为最佳分离方法。The effects of E. coli isolation methods were compared with filtration membrane method, spreading method, and paper sheet method. From the quantity of isolated E. coli from feces it was known that the results of paper sheet method were basically identical to m-TEC medium filtration membrane method. The average quantity of E. coli isolated with m-TEC medium filtration membrane method was 1.4 times that of eosin methylene blue medium spreading method, 2.8 times that of eosin methylene blue medium filtration membrane method, 2.25 times that of m-TEC medium spreading method. Therefore, m-TEC medium filtration membrane method was the best method to isolate E. coli from fecal samples.

关 键 词：大肠埃希菌 滤膜法 涂布法 纸片法 

分 类 号：R378.21[医药卫生—病原生物学]