Theoretical model and quantification of reflectance photometer  

作　　者：黄立华 张友宝 谢承科 屈建峰 黄惠杰 王向朝 

机构地区：[1]Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences

出　　处：《Chinese Optics Letters》2009年第11期1031-1034,共4页中国光学快报（英文版）

摘　　要：The surface morphology of lateral flow （LF） strip is examined by scanning electron microscope （SEM） and the diffuse reflection of porous strip with or without nanogold particles is investigated. Based on the scattering and absorption of nanogold particles, a reflectance photometer is developed for quantification of LF strip with nanogold particles as reporter. The integration of reflection optical density is to indicate the signals of test line and control line. As an example, serial dilutions of microalbunminuria （MAU） solution are used to calibrate the performance of the reflectance photometer. The dose response curve is fitted with a four-parameter logistic mathematical model for the determination of an unknown MAU concentration. The response curve spans a dynamic range of 5 to 200 μg/ml. The developed reflectance photometer can realize simple and quantitative detection of analyte on nanogold-labeled LF strip.The surface morphology of lateral flow （LF） strip is examined by scanning electron microscope （SEM） and the diffuse reflection of porous strip with or without nanogold particles is investigated. Based on the scattering and absorption of nanogold particles, a reflectance photometer is developed for quantification of LF strip with nanogold particles as reporter. The integration of reflection optical density is to indicate the signals of test line and control line. As an example, serial dilutions of microalbunminuria （MAU） solution are used to calibrate the performance of the reflectance photometer. The dose response curve is fitted with a four-parameter logistic mathematical model for the determination of an unknown MAU concentration. The response curve spans a dynamic range of 5 to 200 μg/ml. The developed reflectance photometer can realize simple and quantitative detection of analyte on nanogold-labeled LF strip.

关 键 词：Dynamic response Mathematical models PHOTOMETERS PHOTOMETRY Scanning electron microscopy 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]