通乳丹对CHL细胞株染色体畸变影响的研究  

Effects of Tongru Dan on CHL Chromosomal Aberration

作　　者：刘丹卓[1] 尤昭玲[1] 赵新广[1] 

机构地区：[1]湖南中医药大学,长沙410007

出　　处：《光明中医》2009年第11期2090-2092,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：国家自然基金资助项目(No:30873284);国家教育部高校博士点资助项目(No:20070541001)

摘　　要：目的:探讨通乳丹对CHL细胞株染色体畸变的影响,对其遗传安全性进行研究。方法:采用CHL细胞培养技术,设通乳丹不同剂量对CHL细胞进行体外染毒,观察其致CHL细胞株染色体数目及结构变化。结果:在5000μg/ml、2500μg/ml、1250μg/ml不同测试剂量浓度下,无论24h及48h收集细胞,并在+S9与-S9两种测试条件下,进行染色体畸变分析,各剂量组、溶剂对照染色体畸变率均在正常范围。结论:通乳丹在本试验所确定的5000μg/ml测试剂量浓度以下,初步认为无诱发CHL染色体畸变作用。Objective：By observing the effects of Tong Ru Dan on CHL chromosomal aberration,It is able to open out its genetic toxicity. Methods：The CHL cell was poisoned by Tong Ru Dan in different doses in vitro with cell training techniques,and then,the chromosomal variety of CHL was observed. Results：All the CHL chromosomal aberration rate was normal for Tong Ru Dan with 5000、2500、1250ug/ml,and take timbers after 24h and 48h,mixing into S9 or non-mixing into S9 in all Tong Ru Dan groups. Conclusions：the experiment effects indicate that it has not gene toxicity on Tong Ru Dan in the concentration of 5000ug/ml.

关 键 词：通乳丹 遗传毒性 CHL细胞染色体畸变试验 

分 类 号：R285[医药卫生—中药学]