补肾化痰法对阿尔茨海默病大鼠脑组织CDk5 mRNA表达的影响  

Explore Effect Alzheimer Tau Protein CDk5 mRNA in Rats by Replenishing Kidney-essence and Removing Phlegm Therapy

作　　者：胡慧[1] 王平[1] 孔明望[1] 丁凤敏[1] 

机构地区：[1]湖北中医学院,武汉430061

出　　处：《光明中医》2009年第11期2096-2098,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：国家中医药管理局基金项目(No.04-05JP41);湖北省卫生厅科研基金项目(JX2B66)

摘　　要：目的:观察补肾化痰法对AD模型大鼠CDk5 mRNA表达的影响并探讨其可能机制。方法:应用脑立体定向技术给大鼠BNM注射凝聚态Aβ25-35+IBO构建AD模型。注射2周后,高、中、低量组分别给大鼠该药水煎液灌胃;西药组用哈伯因混悬液灌胃;正常组、空白组、模型组均给予等容积的生理盐水灌胃。28天后采用RT-PCR法检测脑组织CDk5活性。结果:模型组CDk5 mRNA的表达较正常组均有显著性的提高(P<0.01)。给药后发现不但大鼠海马组织Tau蛋白异常磷酸化水平下降,高、中量组与模型组比较显著降低(P<0.01),与西药组也有差异(P<0.01);而且CDk5 mRNA的表达亦明显低于模型组,尤以高量组的作用最明显,高、中、低量组存在一定量效关系(P<0.01)。结论:补肾化痰法可以可能通过抑制CDk5的活性,降低Tau蛋白异常磷酸化水平,从而发挥其抗老年性痴呆的作用。Objective：To explore the effect and potential mechanism of replenishing kidney-essence,removing phlegm therapy on CDk5 mRNA on hyperphos-phorylation for AD rat model. Methods：To induce AD,five groups rats received injection of IBO and Aβ25-35 with basal nucleus of Meynert （BNM） through stereospeetific instrument. After treating for two weeks,BuShen HuaTan decoction were administered by oral gavage for the three doses groups. Huperzine A-Zhulin Antun tablets served as control drug. Normal group, model group and blank group were administered by isovolumetric saline water. After treating for four weeks,RT-PCR was used to detect the activity of cyclin- dependent kinase 5（CDkS）. Results：The expression of CDk5 mRNA in model group have marked increased than normal group（ P 〈 0.01 ）. After administrated by BSHT decoction, not noly the total level of phosphorylation tau protein, but also the expression of CDk5 mRNA have remarkedly increased, especially the high-treated group. Moreover, the high-treated, middletreated and low-treated groups have dose-effect relationship （ P 〈 0.01 ）. Conclusion： Replenishing kidneyessence and removing phlegm therapy can inhibit the activity of CDk5 mRNA and decrease the level of tau protein hyper-phosphorylation.

关 键 词：补肾化痰法 Alzheimer病(AD) 蛋白激酶5(CDk5) TAU蛋白 

分 类 号：R285.5[医药卫生—中药学]