固相萃取-高效液相色谱-荧光检测法测定海水中的多环芳烃  

Determination of Polycyclic Aromatic Hydrocarbons in Seawater Using Solid Phase Extraction-High Performance Liquid Chromatography with Fluorescent Detection

作　　者：李先国[1] 阎国芳 周晓[1,3] 虢新运[1] 王岩[1] 刘金燕[1] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [2]青岛市产品质量监督检验所,山东青岛266061 [3]国家质量监督检验检疫总局,北京100088

出　　处：《中国海洋大学学报（自然科学版）》2009年第5期1087-1092,共6页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(20775074)资助

摘　　要：建立了用固相萃取-高效液相色谱-荧光检测法测定海水中多环芳烃的方法,优化了色谱条件和萃取条件。除苊不能用荧光检测器检出外,其余15种多环芳烃的空白加标回收率在64.5%(苯并[g,h,i],茚并[1,2,3-cd]芘)～88.7%(苯并[a]蒽)之间,相对标准偏差(n=5)为4.9%(荧蒽,苯并[b]荧蒽)～11.1%(苯并[g,h,i],茚并[1,2,3-cd]芘),方法的检出限在0.72(蒽)～14.10 ng/L(荧蒽)之间,基本上达到了痕量分析的要求。利用该方法测得青岛湾表层海水中多环芳烃的浓度在0.125(苯并[k]荧蒽)～25.996 ng/L(萘)之间,但苯并[a]芘未检出。A method using solid phase extraction-high performance liquid chromatography with fluorescent detection was established for the determination of polycyclic aromatic hydrocarbons in seawater; the chromatographic and extraction conditions were optimized. With exception of acenaphthylene which was not fluorescently detected, the recoveries of other 15 PAHs were between 64.5 % （for benzo [g, h, i] perylene and indeno [ 1,2, 3-cd] pyrene） and 88.7 % （for benzo[ a] anthracene）, the relative standard deviations（ n = 5） ranged from 4.9% （for fluoranthene and benzo [b] fluoranthene） to 11.1% （for benzo [g, h, i] perylene and indeno [1,2, 3-cd] pyrene）, and the method detection limits were 0.72 ng/L（for anthracene.） to 14.10 ng/L（for fluoranthene）. The concentrations of polycyclic aromatic hydrocarbons in surface seawater of Qingdao bay area determined by this method ranged from 0. 125 ng/L（for benzo [ k] fluorathene） to 25. 996 ng/L（for naphthalene） while benzo [a] pyrene was not detected.

关 键 词：固相萃取 高效液相色谱 海水 多环芳烃测定 

分 类 号：P734.6[天文地球—海洋化学]