利用SMART技术构建荔枝果皮cDNA文库  

Constructing cDNA Library of Litchi Pericarp Using SMART Technology

作　　者：王家保[1,2] 徐碧玉[2] 金志强[2] 冯超[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《热带作物学报》2009年第8期1109-1112,共4页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30460085);中央级科研院所基本业务费项目(No.ITBB-ZD-2007-3-1);现代荔枝产业技术体系建设专项资金项目资助

摘　　要：以新采收荔枝果实的果皮为材料,利用SMART技术构建荔枝果皮cDNA文库。结果表明,初级噬菌体文库滴度为1.1×106pfu/mL,重组率为88.1%,文库的插入片段平均长度约为850bp;扩增文库滴度为2.6×109pfu/mL。对2444个克隆进行了测序,获得表达序列标签(EST)2136条,平均测序长度562bp。这些EST拼接成为836个簇,含单一序列549条,重叠群287个。初级文库的冗余度为60.8%。A cDNA library of litchi pericarp（Litchi chinensis Sonn.） was constructed with an average insert size of about 850 bp. The titter of primary library was 1.1 ×10^6 pfu/ml and its recombination rate was 88.1% . The titter of amplified library was 2.6 ×10^9 pfu/ml. A total of 2 136 expression sequence tags （ESTs） were obtained by sequencing 2 444 clones from primary cDNA library with an average length of 562 bp. Then 836 clusters were assembled from those ESTs, with 549 singletons and 287 contigs. The redundancy of the primary cDNA library was 60.8%.

关 键 词：荔枝 果皮 CDNA文库 

分 类 号：S667.1[农业科学—果树学]