黄芪多糖对糖尿病仓鼠心肌超微结构和胃促胰酶及血管紧张素转化酶表达的影响  

Effects of astragalns polysaccharides on the myocardial ultrastructure and expressions of chymase and ACE gene in diabetic hamster

作　　者：陈蔚[1] 陈雯洁[1] 叶红英[2] 李益明[2] 

机构地区：[1]复旦大学附属华山医院老年科,上海200040 [2]复旦大学附属华山医院内分泌科,上海200040

出　　处：《中国糖尿病杂志》2009年第10期758-760,共3页Chinese Journal of Diabetes

基　　金：上海市科学委员会科研计划资助项目(05ZR14024)

摘　　要：目的探讨黄芪多糖(APS)对糖尿病心肌病变干预机制。方法检测APS治疗组和糖尿病(DM)对照组仓鼠糖化血清蛋白、胰岛素、C肽、心肌酶谱水平,电镜观察心肌组织超微结构,免疫组化检测心肌Ⅰ、Ⅲ型胶原,放射免疫法测定血浆和心肌血管紧张素Ⅱ(ATⅡ)含量,荧光定量RT-PCR法检测心肌胃促胰酶和血管紧张素转化酶(ACE)表达,放射免疫法检测胃促胰酶和ACE活性,WesternBlot法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。结果与DM组相比,APS组糖化血清蛋白、心肌酶谱、心肌ATⅡ及心肌胶原含量均下降,心肌病变减轻,心肌胃促胰酶表达和活性均下降,心肌p-ERK1/2含量降低,ACE基因表达和活性无统计学差异。结论 APS可抑制糖尿病仓鼠心肌胃促胰酶依赖性ATⅡ生成,从而保护心肌。Objective To investigate effects of astragalus polysaccharides（APS）on diabetic hamster cardiomyopathy. Methods Insulin, C-peptide, myocardial enzymes, glycosylated serum protein（GSP）, AT Ⅱ in plasma and myocardium were tested. Myocardial uhrastructure and collagen were studied. Expressions of chymase and ACE mRNA were detected by real-time fluorescent quantitative RT-PCR, and their protein activities were tested. Expressions of phosphoric extracellular signal-regulated kinase 1/2 （p- ERK1/2）in hearts were measured by Western blot in diabetic hamsters administrated with APS or normal saline（NS）. Results Levels of GSP, CK, CK-MB and myocardial AT Ⅱ in APS-treated group were lower than those in control group. Positive index of myocardial collagen Ⅰ in APS treatment group was less than that in control group. Myocardial ultrastructure was well-protected in APS treatment group compared with control group. Myocardial chymase expression and activity,p-ERK1/2 expression in APS treatment group were lower than those in control group. While levels of insulin, C-peptide, plasma AT Ⅱ and cardial ACE expression and activity were similar in two groups. Conclusions APS is effective in treating diabetic cardiomyopathy due to inhibition of local chymase activity.

关 键 词：黄芪多糖 糖尿病 心肌病变 胃促胰酶 

分 类 号：R285.5[医药卫生—中药学]