深圳地区吸毒人群HIV-1分子流行病学调查  

Molecular epidemiological survey on HIV-1 among the injecting drug users population in Shenzhen

作　　者：赵广录[1] 冯铁建[1] 王晓辉[1] 赵锦[1] 石向东[1] 张燕[1] 甘永霞[1] 罗贤如[1] 

机构地区：[1]深圳市疾病预防控制中心艾滋病防制科,518020

出　　处：《中华流行病学杂志》2009年第11期1131-1134,共4页Chinese Journal of Epidemiology

摘　　要：目的了解HIV-1毒株亚型在深圳地区吸毒人群中的流行情况，并分析其流行趋势及进化规律。方法收集1996—2008年深圳地区吸毒人群HIV确认阳性样本166例，应用巢式聚合酶链反应（nPCR）技术，对样本膜蛋白基因（env）进行扩增，并对其基因区核苷酸序列进行测定和分析。结果166份样本中共存在CRF01_AE、CRF07_BC、CRF08_BC3种重组毒株以及B’、C、A13种亚型；其在所有分析样本中的比例分别为65．06％（108／166）、19．88％（33／166）、6．02％（10／166）、7．23％（12／166）、0．60％（1／166）和1．20％（2／166）；系统进化分析发现，不同时间段HIV-1各亚型出现较明显的汇聚现象；3个不同时间段所流行的CRF01_AE、CRF07_BC亚型组内及与标准参考株间的基因离散率有较为显著性的增大趋势。结论深圳地区吸毒人群HIV-1流行毒株以CRF01_AE重组亚型为主，其次是CRF07_BC重组亚型，也存在CRF08_BC、B’、C、A1亚型，并且各亚型在流行过程中已发生较大变异。Objective To study the prevalent status of human immunodeficiency virus-1 （HIV-1） subtypes in IDU （injecting drug users） population in Shenzhen and to study their source of infection in order to predict the epidemic trend and evolution. Methods 166 HIV-1 positive plasma from the IDUs was collected from 1996 to 2008. HIV-1 env genes were amplified by nested-PCR from RNA. The C2-V3 regions （450 bp） of HIV-1 env were sequenced for analyses. Phylogenetic analyses were performed on the nucleotide sequence data. Results Among 166 samples, there were 6 HIV-1 strains including CRF01_AE, CRF08_BC, CRF07_BC 3 circulating recombinant forms （CRFs） and B＇ ,C, A13 subtypes. Data from the genotype analyses showed that 65,06% （108/166） were CRF01_AE, 19.88% （33/166） were CRF07_BC, 6.02% （10/166） were CRF08_BC, 7.23% （9/166） were subtype B ＇, 0.60% （1/166） were subtype C and 1.20% （2/166） were subtype A1. Phylogenetic tree analysis showed that some of HIV-1 clusters defined in CRF01 AE, CRF07_BC and subtype B＇ in different time groups. Significant increase of gene distance in CRF01_AE and CRF07_BC strains in the three different periods. Conclusion CRF01_AE and CRF07_BC were the major epidemic CRF strains among the IDU population in Shenzhen while the subtype B＇, C, A1 and CRF08_BC were also circulating in IDU population in this region. The variation of all different subtypes was increasing through these years.

关 键 词：艾滋病病毒 吸毒人群 分子流行病学 

分 类 号：R512.91[医药卫生—内科学]