益气止血颗粒中党参炔苷的前处理方法和RP-HPLC含量测定  

Pretreatment and RP-HPLC Determination of Lobetyolin in Yiqi Zhixue Granules

作　　者：郭青[1,2] 孙姗[3] 吕霞[2] 

机构地区：[1]江苏省食品药品检验所,南京210008 [2]中国药科大学,南京210009 [3]南京中医药大学,南京210046

出　　处：《中国药品标准》2009年第5期364-366,共3页Drug Standards of China

摘　　要：目的:建立益气止血颗粒中党参炔苷的含量测定方法。方法:样品采用甲醇超声提取,正丁醇萃取,大孔树脂柱净化,50%乙醇洗脱,C18柱分离,柱温30℃;流动相为水-乙腈(80∶20),流速1.0mL·min-1,检测波长为267nm。结果:党参炔苷能与众多其它组分彻底分离,平均回收率为96.03%(RSD=1.40%,n=6)。平均检测限为16.45ng(RSD=5.89%,n=5),平均定量限为45.31ng(RSD=4.24%,n=5)。结论:所建立的方法专属性强,准确性好,可用于益气止血颗粒中党参炔苷的含量测定和质量标准中党参的质量控制。Objective：To develop an HPLC method for the determination of Lobetyolin in Yiqi Zhixue Granlues. Method：The samples were isonicated with methanol and extracted with n-butanal, the extraction was then cleaned up through resin column in the elution of 50% ethanol. A column of C18 with a temperature at 30 ℃ was used to seperate Lobetyolin, water- aeetonitrile （80： 20） was used as a mobile phase, the flow rate was 1.0 mL · min^ - 1 , the detection wavelength was at 267 nm. Results ： Lobetyolin were seperated perfectly, the average recovery was 96. 03% （ RSD = 1.40%, n = 6）, the average LOD was 16. 45 ng （ RSD = 5.89% ,n = 5 ）, and LOQ was 45.31 ng （RSD = 4. 24% ,n = 5）. Conclusions：The developed method had a good accuracy and specificity,which can be used to determinate Lobetyolin in Yiqi Zhixue Granules, as well as to control the quality of Radix Codonopsis in Yiqi Zhixue Granlues.

关 键 词：益气止血颗粒 党参炔苷 前处理 HPLC色谱法 含量测定 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]