HPLC法测定吡罗昔康片中吡罗昔康的含量  

Determination of piroxican in piroxicam tablets by HPLC

作　　者：吴红英 徐道华 邱栋樑 

机构地区：[1]河南省信阳市食品药品检验所,河南信阳464000

出　　处：《安徽医药》2009年第11期1343-1344,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立HPLC测定吡罗昔康片的方法。方法采用Agilent Eclipse XDB-C18色谱柱,甲醇-0.025 mmol.L^-1磷酸溶液（三乙胺调节pH=3.0）-乙腈（50∶35∶15）为流动相,柱温：22℃,检测波长为334 nm,流速1.0 ml.min^-1。结果吡罗昔康在0.0390-0.7792μg范围内与峰面积呈良好的线形关系（r=1.000 0）;平均回收率为99.00%,RSD=0.41%（n=6）。结论本方法简便、准确、重现性好,可用于该制剂的质量控制。Aim To establish a method for piroxican in piroxican content determination of piroxican in piroxicam tablets by HPLC.Methods A Agilent Eclipse XDB-C18 chromatographic column was used with methanol-0.025 mol·L-1 phosphoric acid（pH adjusted to 3.0 by triethylamine）-acetonitrile（50∶35∶15） as mobile phase at 22℃.The detecting wavelength was 334 nm.The flow rate was 1.0 ml·min-1.Results The linear range was 0.0390～0.7792 μg（r=1.000 0）.The average recovery was 99.00%,with an RSD of 0.41%（n=6）.Conclusion The metnod is simple, accurate and reproducible. It can be used in the quality control of this preparation.

关 键 词：HPLC 吡罗昔康 含量测定 

分 类 号：R927[医药卫生—药学]