纳米CdS∶Cu双酶膜葡萄糖生物传感器  

Study on CdS∶Cu Nano-particles Dual-enzyme Membrane Glucose Biosensor

作　　者：李于善[1] 郭玲芝[1] 李菲菲[1] 徐丽[1] 

机构地区：[1]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《化学世界》2009年第11期657-660,664,共5页Chemical World

摘　　要：将纳米CdS∶Cu颗粒加入到葡萄糖酶（GOD）和辣根过氧化物酶（HRP）双酶膜中,与导电聚合物聚邻苯二胺（PoPD）经电化学聚合反应而固定此两酶,制备了电流型纳米CdS∶Cu颗粒双酶膜葡萄糖生物传感器,分析了CdS∶Cu纳米颗粒对传感器电流响应的影响,进行了传感器的性能测定。实验表明,引入CdS∶Cu纳米粒子和PoPD后可显著改进传感器响应性能,线性范围为0.55-9.2 mmol/L,检测下限为0.55 mmol/L,响应时间为20 s。稳定工作215天,传感器活性指标无显著变化,且抗干扰性强。CdS ： Cu nano-particles were added to the glucose enzyme（GOD） and horseradish peroxidase （HRP） dual-enzyme membrane, and then reacted with conducting polymer poly o-phenylenediamine （PoPD） via electrochemical polymerization to fix these two enzymes and to prepare the current nano-CdS ： Cu particle dual-enzyme membrane glucose biosensors. The response to electric current of CdS ：Cu nanoparticles in the biosensors was analyzed, and the performance of the biosensors was tested. The experimental results showed that the introduction of nano-particles and PoPD significantly enhanced the sensor response sensitivity. The linear range expanded to 0. 55 - 9. 2 mmol/L, and the detection limit reduced to 0.55 mmol/L. The response time was 20 s, During 215 days, the biosensor worked steadily with no significant changes in activity arid exhibited strong anti-interference property.

关 键 词：CdS∶Cu纳米颗粒 生物酶膜 葡萄糖 生物传感器 

分 类 号：O64[理学—物理化学]