直链十二烷基苯磺酸钠对中华倒刺鲃SOD和GSH-Px的活性影响  

Effects of linear alkylbenzene sulfonate(LAS) on the activities of superoxide dismutase and glutathione peroxidase in Spinibarbus sinensis

作　　者：杨帆[1] 孙翰昌[1] 

机构地区：[1]重庆文理学院生命科学与技术学院,重庆402168

出　　处：《淡水渔业》2009年第5期71-75,共5页Freshwater Fisheries

基　　金：重庆市教育委员会科学技术研究资助项目(KJ091219);重庆文理学院重点科研项目资助(Z2007SK14)

摘　　要：以中华倒刺鲃(Spinibarbus sinensis)暴露于不同浓度的直链十二烷基苯磺酸钠(LAS)中10 d,研究阴离子表面活性剂对鱼类抗氧化酶的影响。结果表明,亚致死浓度(4.9 mg/L)LAS暴露可导致中华倒刺鲃超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性发生变化。当LAS质量浓度为0.01 mg/L时,所有受检组织(血液、肝脏)的SOD和GSH-Px活性在暴露初期均受到不同程度的诱导,但LAS浓度为0.2 mg/L和4.0 mg/L和暴露时间超过4 d时,酶活性均呈明显的下降趋势,提示LAS暴露所引起的酶活性变化与暴露浓度和暴露时间有一定的相关性。To study the effects of anionic surfactants on antioxidant enzyme activities in fish, the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were determined in Spinibarbus sinensis after a 10 day exposure to three concentrations （0. 01, 0. 2 and 4. 0 rag/L） of linear alkylbenzene sulfonate （LAS）. The results showed that, investigation of SOD and GSH-Px in plasma and liver indicated that LAS exposure resulted in changes of the enzyme activities. Induction of the two enzymes in all tested tissues occurred in the initial exposure days, and then the enzyme activities decreased with the increase of LAS concentration （0. 2 mg/L and 4. 0 mg/L） and extension of exposure time（4, 6, 8, 10 d）, which suggested that the enzyme activities correlated to the LAS concentrations and the exposure times.

关 键 词：中华倒刺鲃(Spinibarbus sinensis) 直链十二烷基苯磺酸钠 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：S965.9[农业科学—水产养殖] X592[农业科学—水产科学]