腹泻病原菌的分离与鉴定  

Isolation and Identification of Pathogens of Diarrhea

作　　者：黄文智 李淑英[2] 张敏 朱小燕 

机构地区：[1]宜春市人民医院医院感染管理科,江西宜春336000 [2]宜春学院医学院卫生系,江西宜春336000

出　　处：《中华医院感染学杂志》2009年第21期2995-2996,共2页Chinese Journal of Nosocomiology

摘　　要：目的为了提高腹泻病原菌实验室诊断水平。方法利用SS琼脂、山梨醇-麦康凯琼脂(SMAC)、TCBS琼脂、血琼脂之间的功能互补作用,把腹泻粪便同时接种上述4种培养基分离病原菌。结果近3年医院腹泻病原菌检出率达48.2%,分离到的菌种范围不只局限于沙门菌属和志贺菌属,而且致泻性大肠埃希菌、弧菌、金黄色葡萄球菌、白色假丝酵母菌及在平板上呈优势生长的克雷伯菌、变形菌属、柠檬酸杆菌属、铜绿假单胞菌等被陆续检出。结论选择合适的培养基,扩大肠道病原菌的检测范围,在临床微生物检验中非常重要。OBJECTIVE To raise the level of laboratory diagnosis of diarrheal pathogens. METHODS The stool samples were inoculated on the of SS agar, SMAC agar, TCBS agar, blood agar. RESULTS Over the past three years in our hospital pathogen detection rate of diarrhea was 48.2%. The scope of the bacteria isolated were not limited to Salmonella and Shigella, but also Escherichia coli-induced diarrhea, Vibrio, Staphylococcus arreus, Candida albicans. The Klebsiella, Proteus, Citrobacter, Pseudornonasaeruginosa and the like were also detected out. CONCLUSIONS It＇s important to choose right medium and detect intestinal pathogens in clinical.

关 键 词：腹泻 病原菌 细菌培养 

分 类 号：R378[医药卫生—病原生物学]